Histochemical and immunohistochemical studies on peptides and glucosaminoglycans in testes and accesory organs in bulls.

荷斯坦公牛血清抑制素、FSH、LH和睾酮水平的年龄相关性变化：将515头荷斯坦公牛分为0.2、0.5、1、2、3、4、5、6、7和8岁年龄组。采集血样，采集后立即离心分离血清，- 20oc保存至激素检测。血清抑制素、促卵泡激素（FSH）、黄体生成素（LH）水平。放射免疫测定法（RIA）测定各组小鼠的睾酮水平。抑制素血浆水平在1岁前最高（0.2年，2.46ng/ml； 0.5年，2.60ng/ml），随着年龄的增长逐渐降低。与抑制素相比，犊牛1岁前血清FSH水平最低（0.2岁，5.83ng/ml），随年龄增长逐渐升高。血清LH水平随年龄的增长有微弱的上升趋势，但不像FSH那样明显。产后血浆睾酮水平较低，随年龄增长逐渐升高。先前的研究结果明确了抑制素和FSH的年龄相关变化。LH和睾酮。血清抑制素水平在青春期前与FSH呈负相关。出生至青春期荷斯坦公牛犊牛血清抑制素、卵泡刺激素、黄体生成素和睾酮水平、睾丸抑制素和睾酮含量及抑制素的免疫组织化学定位：新生犊牛睾丸抑制素含量最高（3.3ng/g湿重），随年龄增长逐渐降低。8.6月龄的公牛为0.52ng/g。抑制素含量与年龄呈正相关。另一方面，睾酮含量在实验期间没有显示出任何与年龄相关的变化。血清抑制素水平从新生儿期到1.8月龄非常高（7.09ng/g湿重），随着年龄的增长逐渐下降。在8.6月龄的公牛犊牛中，它们是1.98ng/g。抑制素含量与年龄呈正相关。血清FSH和LH水平在1.6月龄时高于其他年龄组，但本研究未发现明显的随年龄增加或减少。血清睾酮水平随着年龄的增长而增加，但与年龄没有明显的相关性。新生儿至2.3月龄犊牛精小管支持细胞抑制素定位阳性反应明显。此后，其强度随年龄增长而降低，6月龄以上未见免疫组化。在6个月以上的小牛的支持细胞中也没有检测到它们。少

Age-Related changes in the Serum Levels of Inhibin, FSH,LH and Testosterone in Holstein Bulls : Five-hundred-fifteen Holstain Bulls were divided into age-groups as 0.2,0.5,1,2,3,4,5,6,7 and 8years old. Bood samples were taken, and sera were separated immediately after collection by centrifugation and stored at -20ﾟCC until hormone assay. Serumlevel of inhibin, folicle stimurating hormone (FSH), luteinizing hormone (LH). and testosterone were assayd by radio immuno assay (RIA). The plasma levels of inhibin were the highest in the young bulls before 1year old (0.2year, 2.46ng/ml ; 0.5year, 2.60ng/ml) and gradually decreased with age. In contrast to inhibin, serum levels of FSH were the low in the young bulls before 1year old (0.2year old, 5.83ng/ml) and gradually increased with ages. The serum levels of LH were suggested a weak tendency to increase with age but not so clear as that of FSH.Plasma levels of testosterone were low at postnatal period and increased gradually with age. The pre … More sent results clatified age-related changes of inhibin, FSH.LH and testosterone. Seru, levels of inhibin were correlated negatively with FSH before the onset of the puberty.Serum Inhibin, FSH,LH and testosterone Levels, Testicular inhibin and Testosterone Contents and Immunohitochemical Localization of Inhibin in Holstein Bull Calves from Birth to Puberty : Teste sinhibin contents were the highest in neonatal calves (3.3ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 0.52ng/g. There was positive correlationship between the inhibin content and age. On the otherhand, testosterone contents did not show any age-related changes in this experimental period.Seruminhibin levels were very high from neonatal period to 1.8month-old calves (7.09ng/g wet weight) and decreased gradually with age. At 8.6month-old bull calves, they were 1.98ng/g. There was positive correlationship between the inhibin content and age. Serum FSH and LH levels were greater at 1.6month-old than other age groups, but clear increrasing or decreasing with age was not found in this sutdy. Serumtestosterone levels were increased with ages, but there was no clear correrationship with age.Positive reactions for inhibin localization were clearly detected in the Sertoli cells of the seminiferous tubules from neonatal to 2.3month-old calves. After that, their intensities were decreased with age, and they were not detected immunohistochemically over 6month-old claves. They were also not detected in any Sertoli cells of over 6month-old calves. Less