Novel enteric nerve plexus links enteric endocrine system with gastrointestinal motility

本研究的主要目的如下：1)自发肌肉收缩起搏器细胞的鉴定。2)控制自发收缩的肠神经系统分析。3)对粘膜刺激的收缩反应。我们得到了四个研究结果。1)起搏器细胞位于狗、豚鼠和小鼠结肠环状肌层粘膜下表面。它们是一种特殊的平滑肌细胞。2)起搏器细胞表达c-kit受体酪氨酸激酶免疫反应性，其发育依赖于c-kit信号通路。3)部分肠神经元表达神经钙蛋白的免疫反应性，神经钙蛋白是一种神经元特异性钙结合蛋白。来源于迷走神经的传入神经末梢也表达神经钙素免疫反应。4)抑瘤基因APC在结肠粘膜上表达。APC产物与β -连环蛋白和DLG共定位。它可能参与粘膜上皮细胞的增殖和分化。这个研究项目是由s.b obayashi作为首席研究员从1995年开始的，在他去世后。鸟桥继续并完成。

The main purpose of this research was as follows ;1) Identification of pacemaker cells of the spontaneous muscle contractions. 2) Analysis of the enteric nervous system which control spontaneous contractions. 3) Contractile response to the stimulation of the mucosa.We obtained four research results.1) Pacemaker cells were located at the submucosal surface of the colonic circular muscle layr in the dog, Guinea-pig and the mouse. They were a kind of special smooth muscle cells.2) Pacemaker cells expressed c-kit receptor tyrosine kinase immunoreactivity and their development depended on c-kit signaling pathway.3) Some enteric neurons expressed immunoreactivity of neurocalcin which is a neuuron specific calcium binding protein. Afferent nerve ending originated from vagus also expressed neurocalcin immunoreactivity4) The tumor suppressor gene APC was expressed on the mucosa of the colon. The products of APC were co-localized with beta catenin and with DLG.It may be involved in the proliferation and differentiation of mucosal epithelial cells.This research project was started by S.kobayashi as a head investigator from 1995 and after his death. S.Torihashi continued and completed.