PREDICTION OF CAUSATIVE AGENTS AGAINST OCCUPATIONAL HYPERSENSITIVITY PNEUMONITIS AND DETERMINATION OF THE THRESHOLD LIMIT VALUE

1）建立了一种动物实验模型，用于预测职业环境中产生的物质，特别是真菌对过敏性肺炎（HP）的致敏性。最后，我们通过反复鼻腔给药悬浮在蒸馏水中的真菌抗原，在c57 Black/6小鼠肺组织中建立HP。从塑料大棚种植茄子中分离纯化培养的镰刀菌（Fusarium sp.），经5次共5周的暴露后，动物肺组织出现典型的HP病变。结果表明，本试验模型可用于检测对HP过敏的工作场所中的真菌，并首次证实了镰刀菌的HP过敏性sp.2）制定并提出了职业环境尤其是农业环境中接触材料的HP过敏性预测检测及预防的流程图，包括本研究项目开发的动物实验模型。我们阐明了茄子组分提取物的立即型过敏原性和塑料温室栽培的Alstromeria提取物的延迟型过敏原性，在种植这些作物的工人中发现了过敏病例。

1)We conducted to establish an animal experimental model for predictive assessment of an allergenecity of hypersensitivity pneumonitis (HP) against materials born in occupational environment, especially fungi. Finally we adopted for development of HP in the lung tissue of c57Black/6 mice by repeated nasal administration of fungus antigen suspended in distillled water. The test fungus was Fusarium sp. sampled from the plastic greenhous planting eggplants and isolated by purified cultivation.The treated animals developed typical HP lesions in their lung tissue after total of 5 times exposure for 5 weeks.The result suggested that the trial model was useful and adequate to detect fungi sp. in the working places showing allergenesity to HP and also demonstrated firstly the allergenesity of HP of Fusarium sp.2)We made and proposed the flow chart for predictive ditection of allergenesity of HP and it's prevention among materials born in occupational environment, especially in agriculture, including the animal experimental model developed in the present research project.3)We clarified an immediate type allergenesity of an extract from eggplant component and a delayd type allergenesity of an extract of alstromeria both cultivated in plastic greenhouse and found allergic cases in workers of those cultivations.