Possible reasons for immunological tolerance in rat liver transp lantation

在从Lewis(Lew)到Wistar Furth(WF)大鼠的原位肝移植中，发生了一过性的急性排斥反应，随后发生了自发的接受。在先前的研究中，我们发现在长期存活的WF大鼠中，预置的细胞毒性T淋巴细胞前体(CTLp)被消除，进一步的研究表明，这些动物的Lew肝移植物没有同种异体刺激活性。在这项研究中，我们研究了移植物的免疫刺激活性以及移植物中移行的供体细胞到受体脾的动态变化。同种异体肝移植后第1天和第2天观察到同种异体刺激活性，第3天基本消失。受体WF大鼠肝移植后12小时获得的脾细胞对LEW有较强的同种异体刺激作用，而第3天的脾细胞在体内未观察到这种反应。

In orthotopic liver transplantation from Lewis (LEW) into Wistar Furth (WF) rats, acute rejection response occurred transiently, followed by the spontaneous acceptance of the graft. In the previous study, we have found that primed cytotoxic T lymphocyte precursors (CTLp) were eliminated in long-term surviving recipient WF rats, and furthe rmore showed that LEW liver grafts from those animals had no allostimulatory activity. In this study, we investigated kinetic change in immunostimulatory activity of the graft and of the migrating donor cells from the graft into the recipient spleen. Allostimulatory activity of the liver graft was observed on days 1 and 2 and disappeared by day 3 after the trabsplantation. The spleen cells from recipient WF rats obtained 12 hours after liver transplantation showed potent allostimulatory capacity of LEW,but no such response was observed in vivo with the spleen cells obtained on day 3.