Development of Multi-frequency Vector EPR Spectrometer by White-noise Field Modulation Method.

综述了矢量电子自旋共振波谱仪的发展和应用。矢量ESR是在饱和转移ESR方法的基础上发展起来的。我们将该方法应用于各种生物材料系统，发现矢量ESR为连续波区的选择性检测提供了一种通用的方法。我们将进一步指出矢量ESR在生物分子动力学研究中的潜力。到目前为止，矢量ESR光谱在生物学上的应用才刚刚开始，主要集中在蛋白质和生物膜系统这两个领域。Vistnes利用ST-ESR检测的相位信号研究了自旋标记血红蛋白的动力学。Watari等人。论证了矢量ESR波谱在黄素酶体系中应用的可行性，并对相谱的灵敏度进行了分析。它们还表明，幅度谱和相位谱都可以检测到来自铁血红蛋白系统的未知高自旋信号。

Development and applications of Vector ESR spectroscopies were reviewed. Vector ESR was developed on the basis of saturation transfer ESR method. We applied the method on the various biomaterial systems, and found that Vector ESR gives a universal method for selective detection in the continuous wave regime. We will further indicate the potentiality vector ESR to the study of biomolecular dynamics.Thus far, biological applications of vector ESR spectroscopy are just the beginning, and mainly focused on the two area, namely protein and biomembrane systems. Vistnes studied dynamics of spin-labeled hemoglobin using the phase signal of ST-ESR detection. Watari et al. Showed the feasibility of vector ESR spectroscopy as applied on the flavin enzyme system, and found the sensitivity of the phase spectrum. They also indicated that both the amplitude and phase spectra can detected the unknown high spin signal from ferrihemoglobin system.