Developement of rotating annular chromatographic reactor for continuous production of pectine oligosaccharide

为了开发一种连续生产果胶低聚糖的旋转环状层析反应器，有必要研究旋转环状层析的分离性能和固定化酶连续解聚的动力学。在不同的初始底物和酶浓度条件下，分别用悬浮酶和固定化酶对低聚糖进行了水解实验。选择聚合度为6的低聚糖异麦芽糖作为底物，因为连续解聚的机理比较简单，且浓度易于分析。采用内切-葡聚糖酶作为酶制剂。通过引入酶对底物每个α-1，6-糖苷键的选择性，建立了一个新的悬浮酶体系动力学模型。选择性系数估计为0.411，与Exper…的结果基本一致更具内涵的价值观。通过考虑颗粒内的传质阻力，将该模型推广到固定化酶体系。将扩展模型与各种条件下的实验数据进行拟合，得到了模型常数，固定化酶体系的动力学常数远大于悬浮酶体系的动力学常数。载体颗粒中有效扩散系数的估计值比水溶液中的小几个数量级。设计并制备了固定化酶填充柱反应器。利用柱式反应器，在不同的流速、底物浓度和酶浓度条件下进行了连续水解实验。在固定化酶系统动力学模型的基础上，建立了连续过程的数学模型。用模型常数估计值对固定化酶体系进行了模拟，模拟结果与实验结果吻合较好。较少

In order to develope a rotating annular chromatographic reactor for continuous production of pectin oligosaccharide, it is necessary to clarify the separation performance of the rotating annular chromatography and the kinetics of the consecutive depolymerization using the immobilized enzyme. The hydrolysis experiments of oligosaccharide using both suspended and immobilized enzymes were carried out under various conditions of the initial substrate and enzyme concentrations. Isomaltohexaose, the oligosaccharide of the polymerization degree of 6, was chosen as a substrate, because the mechanism of consecutive depolymerization was comparatively simple and the concentration could be easily analyzed. Endo-dextranase was used as a enzyme. A novel kinetic model for the suspended enzyme system was developed by introducing the selectivity of the enzyme for cleaving each alpha-1,6-glycosidic bond of the substrate. The selectivity coefficient was estimated at 0.411 and almost the same as the exper … More imental values. The model was extended for the immobilized enzyme system by taking into account the intraparticle mass transfer resistance. The model constants were estimated by fitting the extended model with the experimental data obtained under the various conditions.The kinetic constants for the immobilized enzyme system are much larger than those for the suspended enzyme system. The estimated values of effective diffusivities in the support particle were a few order of magnitude smaller than those in aqueous solution. A column reactor packed with the immoblized enzyme was designed and prepared. The experiments of the continuous hydrolysis were carried out using the column reactor under the various conditions of the flow rate, the substrate concentration and the enzyme concentration. A mathematical model for the continuous process was constructed on the basis of the kinetic model for the immobilized enzyme system. The simulated results by the model using the estimated values of the model constants in the immobilized enzyme system were in good agreement with the experimental results. Less