Efficient production and in vivo-effect of promoters of nerve growth factor-synthesis from edible mushrooms.

1)从蘑菇中大规模制备已知活性化合物和寻找新的活性化合物。部分蘑菇品种（10 ~ 500kg）采用乙醇提取。去除乙醇后，将萃取物在乙酸乙酯或氯仿等有机溶剂和水之间分离。有机溶剂溶性部分通过重复色谱分离，得到已知的活性化合物用于体内测定。此外，通过对一些蘑菇提取物的活性筛选，发现一些蘑菇能促进ngf的产生。结果表明，从工业双孢菌中分离得到了两个新的化合物：双孢菌碱a和B。2)高效生产活性化合物。在不同的培养条件下，考察了野味菌A的产量。结果，发现了一个有效的条件（3g在100 ml培养基中）。3) hericenones和erinacines的体内实验。从蘑菇Hericium erinaceum中分离出了ngf合成的启动子hericenones和erinacines。用模型大鼠研究了这些化合物的体内作用。目前正在对影响进行评估。

1) Large scale preparation of known active compounds and search for novel active compounds from mushrooms.Some kinds of mushrooms (10-500kg) were extracted with ethanol. After removing the ethanol, the extracts were partitioned between organic solvents such as ethyl acetate or chloroform and water. The organic solvent-soluble parts were separated by repeated chromatography, giving known active compounds for in vivo assay. In addition, by screening of the activity against some mushroom extracts, it was revealed that some mushrooms promoted NGF-production. As a result, two new compounds, dictyophorine A and B were purified from a mushroom Dictyophora indusiata.2) Efficient production of active compounds.Production of erinacines A were examined under various culture conditions. As a results, an efficient condition was found (3g in 100 ml medium).3) In vivo experiments of hericenones and erinacines.The promoters of NGF-synthesis, hericenones and erinacines, have been isolated from a mushroom Hericium erinaceum. The in vivo effects of these compounds were examined using some model rats. The valuation of the effects is now in progress.