A Study on Production of a New Model of Cleft Lip and Cleft Palate by Fetal Manipulation

我们进行了实验上的生产的动物模型，以澄清颅面畸形，包括唇腭裂的发育因素和模式的进展，面部畸形与畸形在胎儿时期。在小鼠胎儿中进行子宫外手术。在胎龄10-12天时，当胎儿面部的基本形态完成时，通过使用细玻璃针电极的电凝或氩激光光凝来损伤面部原基，在随后的生长期间诱导面部变形。通过这些程序，我们寻找一种胎儿操作方法来阐明从胚胎面部投影形成到出生的面部生长模式. 11-12日龄的胎面直接受蜕膜、卵黄囊和羊膜侵犯的胎儿，其生长发育与周围宫内胎儿相似.在使用细玻璃针电极电凝侵入面部的子宫外胎儿中，约50%存活至胎龄12天，并显示不对称的面部和深部继发性腭组织缺损。在妊娠11天时面部受到氩激光光凝侵犯的子宫外胎儿中，20%存活至足月，主要表现为由于面部表层组织缺陷导致的面部形态不对称。CL/Fr小鼠群体中发生的以上颌骨不对称生长为特征的畸形被确定为遗传亚系。这些结果表明，胎儿面部操作可以诱导先天性颅面畸形。本研究可为实验系统的建立提供有用的数据。

We carried out experiments on the production of an animal model for clarification of the developmental factors of craniofacial malformations including cleft lip and palate and the mode of progression of facial deformations associated with malformations during the fetal period.1. Exo utero surgery was performed in mouse fetuses. At a fetal age of 10-12 days when the basic morphology of the fetal face is completed, the face primodium was injured by electrocoagulation using fine glass needle electrodes or argon laser photocoagulation, inducing facial deformations during subsequent growth. By these procedures, we searched for a fetal manipulation method to clarify the mode of facial growth from the formation of the mouse embryonic facial projection to birth.2. Exo utero fetuses in which the face was directly invaded via the decidua, yolk sac, and the aminon at the age of 11-12 days subsequently showed growth similar to that in the surrounding intrauterine fetuses.3. Of exo utero fetuses in which the face was invaded by electrocoagulation using fine glass needle electrodes, about 50% survived to a fetal age of 12 days and showed asymmetrical face and secondary palatal tissue defects in the deep area.4. Of exo utero fetuses in which the face was invaded by argon laser photocoagulation on 11 days of gestation, 20% survived to the term and primarily showed asymmetrical facial morphology due to tissue difects in the facial surface layr.5. The malformation characterized by maxillary asymmetrical growth that occurred in a CL/Fr mouse colony was established as a genetic substrain.These results suggest that congenital craniofacial deformations can be induced by fetal face manipulation. This study may provide useful data for the establishment of experimental systems.