Genetical analysis of sexual behavior in Drosophila melanogasteranalyzes

为了了解物种形成的过程、神经系统的组织和性别决定的机制，我分析了黑腹果蝇的交配行为突变。构建了4000个P元素标记品系，从中筛选出不育或半不育品系。每个不育品系都进行了交配行为测试，因为具有严重交配行为缺陷的文件看起来像不育。我从140个无菌菌株中获得了几个行为突变候选菌株。933号品系的雌性有异常的生殖器，令人惊讶的是，它们试图与雄性求爱，而不是雌性。更详细的分析表明，该突变是Transform-2的一个亚型等位基因。3个具有明显表型和雄性求偶行为缺陷的突变群体被鉴定为先前特征基因的新等位基因。其中一个这样的突变是前开放(AOP)的等位基因，这种突变有粗糙的眼睛和脱色的老虎。另外两种是粗糙(SCA)和轮腹(RT)等位基因。从上述P标记品系中获得37个雌性交配行为突变候选者。每个突变体在交配试验中都表现出很高的重复性。

To understand the process of speciation, organization of nervous system and mechanism of sex-determination, I have analyzed mating behavior mutations in Drosophila melanogaster. 4000 P-element tagged lines were constructed and sterile or semi-sterile stocks were selected from them.Each sterile strain was tested for its mating behavior, because files with severe mating behavioral defects look like sterile. I obtained several behavior mutation candiates out of 140 sterile strains. Females of the strain #933 have abnormal genitalia and surprisingly they try to court with males but females. More detailed analysis showed that this mutation was a hypomorphic allele of transformer-2. Three mutant stocks with visible phenotype as well as courtship behavior defect in males were identified as new alleles of previously characterized genes. One such mutation was an allele of anterior-open (aop) and this had rough eyes and depigmented tergites. Other two stockes are alleles of scabrous (sca) and rotaed-abdoment (rt). Using males of a D.simulans stock, 37 female mating behavior mutation candidate were obtained from above P-tagged lines. Each mutant showed high repeatability for their mating tests.