Observed Cloning Gene on Mitotic Chromosomes at that number in Basidiomycetes

采用等高夹持均匀电场（CHEF）凝胶电泳和DNA探针氢化技术对金针菇（Flammulina velutipes, Curt: Fr.） Sing的核型进行了电泳分析。单核体（Fv-4K）和双核体（Fv-4）的染色体DNA分别被分解成6条和8条条带。染色体大小在1.9 ~ 6.0兆碱基对之间。每个分离的染色体DNA带被鉴定和分配使用五个克隆探针。这些染色体的数量估计分别为6条和12条，整个基因组的大小估计分别为20.1 Mb和38.6 Mb。通过模式比较和杂交分析，我们推断出Fv-4菌株存在不同大小的等位染色体。用4′，6-二氨基-2-苯基吲哚（DAPI）荧光染色剂对利用子孢子爆发法进行有丝分裂的平菇（Pleurotus cornucopiae (Paulet) Rolland var. citrinopileatus）染色体进行了染色，但染色体的组成不能明显区分。因为染色体是非常小的样本。

The karyotype of Flammulina velutipes (Curt : Fr.) Sing was analyzed electrophoretically by contour-clamped homogeneous electric field (CHEF) gel electrophoresis and hydridization with DNA probe.The chromosomal DNA from the monokaryon (Fv-4K) and the dikaryon (Fv-4) were resolved into six and eight bands, respectively.The sizes of the chromosomes were ranged from 1.9 to 6.0 megabase pairs. Each of the separated bands of chromosomal DNA was identified and was assigned by use of five cloned probes.The number of these chromosomes was estimated to be six and twelve, respectively, and the size of the entire genome was estimated to be about 20.1 Mb and 38.6 Mb, respectively.From comparison of patterns, and hybridization, we deduced the existence of allelic chromosomes of different sizes in the Fv-4 strain. Mitotic chromosomes of Pleurotus cornucopiae (Paulet) Rolland var. citrinopileatus by the Basidium burst method were stained with the fluoresent dye 4', 6-diamidino-2-phenylindole (DAPI), but the component of chromosomes were not clearly distingushed. because chromosomes were very small specimens.