Isothermal microcalorimeter

等温微量热法是一种非常通用的分析方法，用于表征涉及热转换的过程。它可以采用各种形式，以静态的方式，以及，例如，通过控制引入反应物。在模块化设计的帮助下，这里应用的仪器旨在实现静态等温量热法以及滴定法（等温滴定量热法）和监测溶解或混合过程的测量（溶液量热法），温度与各自的应用相关。它将被布伦瑞克工业大学制药、化学和工艺工程研究所的工作小组使用，这些研究小组在其制药工程中心进行跨学科研究。该设备的计划应用区域相应广泛，测量设置的要求也是如此。大多数应用是在制定具有苛刻物理化学性质的活性药物成分的策略领域。本研究的一个重要方面是对配方及其与活性成分和生物模型系统的相互作用进行全面的物理化学表征。除其他外，申请的设备将用于研究制剂的长期稳定性和物理状态，活性成分和赋形剂与胶体和纳米结构载体系统及其组分的相互作用，以及胶体制剂与生理（模型）流体和细胞培养模型的相互作用。静态等温量热法将用于监测长期的物理过程（例如，结晶、多态转变、相分离），并最终监测化学变化。这种方法也将用于研究细胞培养和分离细胞系统的代谢。通过等温滴定量热法，纳米颗粒系统与药学上相关的结合伙伴的相互作用，以及在更技术导向的系统中，以及溶液中（模型）生物分子之间的相互作用，将被研究。溶液量热法将用于确定基本参数，以确定药物的溶解度和固体状态在药学相关的赋形剂系统。该实验装置还将允许研究赋形剂系统与生理液体的相互作用以及赋形剂溶液与药用固体的相互作用。

Isothermal microcalorimetry is a very versatile analytical method for characterizing processes involving heat conversion. It can be employed in various forms, in a static way as well as, for example, via the controlled introduction of reactants. With the aid of a modular design, the instrument applied for here is intended to enable static isothermal calorimetry as well as titrations (isothermal titration calorimetry) and measurements for monitoring dissolution or mixing processes (solution calorimetry) at temperatures relevant for the respective applications. It is going to be used by working groups from the pharmaceutical, chemical and process engineering institutes of the TU Braunschweig, which conduct interdisciplinary research in its Center of Pharmaceutical Engineering. The planned areas of application for the device are correspondingly extensive, as are the requirements for the measurement setup. Most applications are in the field of developing strategies for formulating active pharmaceutical ingredients with demanding physicochemical properties. An essential aspect of this research is the comprehensive physicochemical characterization of the formulations and their interactions with active ingredients and biological model systems. The device applied for will be used, among other things, to investigate the long-term stability and physical state of formulations, the interactions of active ingredients and excipients with colloidal and nanostructured carrier systems and their components, and the interactions of colloidal formulations with physiological (model) fluids and cell culture models. Static isothermal calorimetry will be used to monitor long-term physical processes (e.g., crystallization, polymorphic transformations, phase separation) and eventually also chemical changes. This method will also be used to study cell cultures and the metabolism of isolated cell systems. By means of isothermal titration calorimetry, interactions of nanoparticulate systems with pharmaceutically relevant binding partners, but also in more technically oriented systems, as well as between (model) biomolecules in solution, will be investigated. Solution calorimetry will be used to determine basic parameters for the determination of drug solubilities and the solid states in pharmaceutically relevant excipient systems. This experimental setup will also allow the investigation of the interaction of excipient systems with physiological fluids and the interaction of excipient solutions with pharmaceutical solids.