MRI: Acquisition of an Automated Isothermal Titration Microcalorimeter for the Emory Macromolecular Structure and Interactions Suite

MRI：为埃默里大分子结构和相互作用套件购买自动等温滴定微量热计

• 批准号: 1040177
• 负责人: Graeme Conn
• 金额: $ 18.1万
• 依托单位: Emory University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1040177&HistoricalAwards=false
• 关键词: MRI; Acquisition; Automated; Isothermal; Titration

This Major Research Instrumentation award funds the acquisition of an automated small cell isothermal titration microcalorimeter for the Emory University Department of Biochemistry's "Macromolecular Structure and Interactions Suite." A long-term goal for the assembled team of investigators is to understand the biochemical basis for macromolecular interactions using a combination of structural biology and biophysical techniques. The new microcalorimeter enhances Emory's diverse research programs that include the investigations of protein synthesis regulation by the ribosome; protein, DNA and RNA modifications in epigenetics, protein sorting, protein degradation and antibiotic resistance; lipid transport; and nuclear receptor function. Understanding the biophysical parameters that govern macromolecular interactions adds a complementary dimension to Emory University structural biology programs. The new equipment therefore enhances training in biophysical experimental methods by improving access to state-of-the-art instrumentation for post-doctoral fellows, graduate students and undergraduates. Furthermore, high school students and teachers will get hands-on experience in a joint Emory-Georgia Tech summer program designed to study the biophysical characterization of extremophile ribosomes. The results of these research and teaching efforts will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.

这项重大研究仪器奖资助埃默里大学生物化学系的“大分子结构和相互作用套件”购买一台自动化小细胞等温滴定微量热仪。“一个长期的目标，为组装团队的研究人员是了解生物化学基础的大分子相互作用的结合使用结构生物学和生物物理技术。 新的微量热仪增强了埃默里大学的多样化研究计划，包括核糖体对蛋白质合成调控的研究;表观遗传学中的蛋白质，DNA和RNA修饰，蛋白质分选，蛋白质降解和抗生素抗性;脂质转运;和核受体功能。 了解支配大分子相互作用的生物物理参数为埃默里大学的结构生物学课程增加了一个补充维度。 因此，新设备通过改善博士后研究员、研究生和本科生获得最先进仪器的机会，加强了生物物理实验方法的培训。 此外，高中学生和教师将获得实践经验，在一个联合埃默里佐治亚理工学院的夏季计划，旨在研究极端核糖体的生物物理特性。 这些研究和教学工作的结果将通过摘要和同行评议的出版物，以及学生和教师在专业会议的积极参与广泛传播。