Studies on Structure and Fanction of Staphylococcal Enterotoxin and Bacillus cereus emetic toxin

葡萄球菌肠毒素（SEs）血清学分为A、B、C五种类型：C_1、C_2、C_3、D和E。这些研究尚未完全了解SES的生物学和功能活性位点的分析。我们尝试研究SE-A的抗原决定因子（表位）。所得结果如下：1。天然SE-A分子含有至少3种不同的抗原决定因子（合成肽A-1：氨基酸残基1-20，A-5: 81-100和A-8: 141-160）。SE-A分子上的氨基酸残基161-180是小鼠产生INF-的重要区域。为研究呕吐型食物中毒爆发分离的蜡样芽孢杆菌产生的HEp-2液泡活性与呕吐毒素的关系，测定了纯化的HEp-2因子（Cereulid）对恒河猴和猕猴的呕吐活性。所得结果如下：1。纯化后的Cereulide （HEp-2因子）被强烈认为是一种催吐毒素。Cereulide在消化道中稳定，可引起呕吐，并表现出导致细胞损伤的致死活性。

The staphylococcal enterotoxins (SEs) are serologically classified in five types(A,B,C : C_1, C_2, C_3, D,and E). It was not yet fully understood by these studies to anaylyze the biologically and functionalyy active sites of SES.Attempts were made to investigate the antigenic determinants (epitopes) of SE-A.The results obtained were as follows.1.Native SE-A molecule contains at least 3 different antigenic determinants (synthetic peptides A-1 : amino acid residues 1-20, A-5 : 81-100 and A-8 : 141-160).2.Amino acid residue 161-180 on SE-A molecule is an essential region for production of murine INF-.To study the correlation between emetic toxin and HEp-2 vacuole activity produced by Bacillus cereus isolated from an outbreak of vomiting type food poisoning, emetic activities of purified HEp-2 factor (Cereulid) to rhesus monkeys and suncus were determined. The results obtained were as follows.1.The purified Cereulide (HEp-2 factor) is strongly suggested to be an emetic toxin itself.2.The Cereulide is stable in the digestive tracts, induce emesis, and show lethal activity leading to be cellular damage.