Cellular dynamics in the movement of hematopoietic foci

我们对胎鼠肝脏进行了分析，以研究造血运动的机制：在哺乳动物中，个体发育的造血灶从卵黄囊转移到胎肝，最终骨髓成为成年大鼠的主要造血部位。目前尚不清楚为什么会发生造血活动。为了分析胎肝的变化，我们提出了单抗，并获得了UB11和UB12，它们标记了大部分胎肝造血细胞和大约30%的成人骨髓细胞(Fujikura等，1990)。在本研究中，我们还获得了另外三种抗胎肝细胞的单抗(UB18、HAM10和HAM11)。UB18标记了少数胎肝造血细胞和成人骨髓细胞。UB18识别的分子的性质尚不清楚。HAM10和HAM11识别胎肝细胞线粒体和内质网中的一些分子。强大的美联社…当胎肝造血广泛时，HAM10抗原出现较多，而当造血开始消失时，则以HAM11抗原为主。因此，我们正在研究HAM10和HAM11的抗原表达变化与造血的关系(Love et al.，1996)。我们还分析了辐射后再生的胸腺，因为6GY照射后胸腺缩小了尺寸和产生T细胞的功能，但恢复得很快。例如，照射后第5天，胸腺细胞数量是第3天的10倍，皮质-内侧边界明显。同时，我们认为在照射后胸腺细胞数量减少后，胸腺组织中可能存在一些相当强大的突发性支持间质功能。为了研究再生胸腺的变化，我们分析了胸腺向胸腺注入印度墨水时的血管变化，以可视化血管结构。结果表明，血管结构对胸腺的突然再生具有重要意义。我们还使用胸腺因子抗体对胸腺上皮细胞进行了免疫组织化学分析。结果表明，在恢复期可以观察到FTS的密集染色。这些数据可能支持胸腺细胞在再生胸腺中的突然增殖。较少

We have analyzed fetal rat liver to study the mechanism responsible for the movement of hematopoiesis : In mammals, ontogenically hematopoietic foci move from yolk sac to fetal liver and eventually bone marrow becomes a predominant site for the hematopoiesis in adult. It is not know why hematopoiesis moves. In order to analyze the changes in fetal liver we have raised monoclonal antibodies and obtained UB11 and UB12 which label most cells in fetal liver hematopoietic cells and about 30% of adult bone marrow cells (Fujikura et al., 1990). And we used these to analyze the changes of hematopoiesis in fetal liver.In this study, we could get three other monoclonal antibodies to fetal liver cells (UB18, HAM10 and HAM11). UB18 labelled a few percent of fetal liver hematopoietic cells and adult bone marrow cells. The nature of the molecules which UB18 recognises are not known. HAM10 and HAM11 recognise some molecules in mitochondria and endoplasmic reticulum of fetal hepatocytes. The strong ap … More pearance of HAM10 antigen was observed when hematopoiesis are extensive in fetal liver, while HAM11 antigen was dominant when hematopoiesis started to disappear. Thus, we are on the way to study the relationship between the antigen expression changes of HAM10 and HAM11 and hematopoiesis (Lovely et al., 1996).We have also analyzed the regenerating thymus after irradiation, as the thymus reduces its size and function of T cell production after receiving 6Gy irradiation, while it recovers quite quickly. For example, on day 5 after irradiation thymocytes number increases 10 times of that of day 3, and on day cortico-meduallary boundary becomes obvious. Susequently, we considered that after irradiation and diminution in number of thymocytes, some quite potent abrupt supporting stromal function may work in thymic tissue. To study the changes in regenerating thymus, we analyzed the vascular changes of the thymus injecting Indian ink into the thymus to visualise the vascular structures. Results suggest that vascular structures were important for the abrupt thymic regeneration. We also analyzed thymic epithelial cells immunohistochemically using antibodies to thymic factors as FTS.Results suggest that the intensive staining of FTS was observed during the time of recovery. These data may support the adrupt proliferation of thymocytes in regenerating thymus. Less