Study on the pathophysiological mechanism of the sleep-wake rhythm disorder caused by the phase change of LD cycle.

本研究的目的是阐明“时差综合征”睡眠-觉醒节律紊乱的病理生理机制。本实验连续两周同时记录大鼠的警觉状态、体温（Tb）、血压（BP）和心率（HR），并进行了以下研究：1）在普通12-12小时光暗（LD）循环下，观察各参数的昼夜节律及其相互作用;（2）在8小时LD周期相位提前下观察各参数的日节律变化及其相互作用;（3）观察维生素B12对2）中所获得的日节律变化的影响。结果表明：1）尽管HR和Tb在所有警戒状态下均表现出类似的正弦日变化，但BP的日曲线依赖于警戒状态。清醒期（W），暗相血压昼夜变化较大，非快速眼动睡眠期（NREMS）血压昼夜变化不明显，快速眼动睡眠期（REMS）血压昼夜变化不明显。 ...更多信息 相反的昼夜变化，即，在亮相期间比暗相期间高。（2）LD周期的相位提前导致NREMS和REMS均增加，相位提前后NREMS的昼夜幅度降低，但REMS的昼夜幅度暂时增加，相位提前后4-5 d内REMS的日型几乎不变。在相位提前后的4-5天内，光期与前一暗期的NREMS数量呈显著正相关，说明相位提前干扰了NREMS数量的自稳控制。Tb的昼夜节律幅度也从相位提前后开始下降。所有警觉状态下血压和心率的昼夜节律均无明显变化; 3）渗透泵持续腹腔内输注维生素B12抑制LD周期相位提前后睡眠的增加。维生素B12可以有效地预防“时差综合症”。少

The aim of this study was to clarify the pathophysiological mechanisms of sleep-wake rhythm dysfunction in "jet-lag" syndrome. I have recorded rats' vigilance state, body temperature (Tb), blood pressure (BP) and heart rate (HR) simultaneously for about 2 weeks, and done the following studies : 1) the observation of the diurnal rhythm in each parameter and its interaction under the ordinary 12-12 hours light-dark (LD) cycle ; 2) the observation of the change of the diurnal rhythm in each parameter and its interaction under an 8-hour LD cycle phase advance ; 3) the observation of effect of vitamin B12 on the change of diurnal rhythm obtained in 2). As a result, 1) although HR and Tb showed a similar sinusoidal diurnal variation in all vigilance states, the diurnal profile for the BP was dependent on vigilance state. During wake (W), the BP diurnal variation was higher during the dark phase, during NREM sleep (NREMS), no significant diurnal change was seen, while during REMS,the BP exhib … More ited reversed diurnal variation, i.e., it was higher during the light phase than the dark phase. The phasic fluctuation of BP during REMS was greater in the light phase than the dark phase ; 2) phase advance of the LD cycle led to an increase in both NREMS and REMS.Although the circadian amplitude of NREMS decreased just after the phase advance, that of REMS temporarily increased, and REMS diurnal pattern hardly changed for 4-5 days after phase advance. The amount of NREMS in the light period correlated positively with that in the preceding dark period for 4-5 days after phase advance, indicating that homeostatic control of the amount of NREMS was disturbed by phase advance. The circadian amplitude of Tb also decreased from just after phase advance. There was no large change in the diurnal rhythm of BP and HR in all vigilance state ; 3) continuous intraperitoneal infusion of vitamin B12 by an osmotic pump suppressed the increase of sleep after the phase advance of LD cycle. Vitamin B12 can be effective in prevention of the "jet-lag" syndrome. Less