ION TRANSPORT,LIPID METABOLISM AND HEAT PRODUCTION DURING COLD ACCLIMATION IN RAT BROWN ADIPOSE TISSUE CELLS.

本文研究了冷、温（8 w）驯化和再驯化（冷，4 w：温，4 w）对棕色脂肪组织细胞内离子转运、脂代谢和产热的影响。用电子探针X射线微区分析和膜片钳技术观察细胞器，高压电镜观察显示冷习服大鼠组织细胞线粒体肿胀，脂滴减少和/或变形。X射线微区分析表明，冷驯化的细胞线粒体中K和Cl含量较低，Ca含量较高。再习服组大鼠的Ca下降到热习服组大鼠的水平。用膜片钳技术研究冷适应过程中细胞器重构膜的电压和/或H+敏感电流。实验结果表明，在冷驯化过程中，细胞器的内膜中存在一个小的和一个相对较大的电导，对应于Cl-通道和Ca ~（2+）依赖的K ~+通道。

The effects of warm or cold (8w) acclimation and reacclimation (cold, 4w : warm, 4w) on the intracellular ion transport, lipid metabolism and heat production of the brown adipose tissue cells were studied. The cells were investigated by electron probe X-ray microanalysis and organelles by the patch clamp technique.The high voltage electron micrographs of the cells of cold acclimated rats revealed mitochondrial swelling and a decrease and/or deformation of lipid droplets of tissue cells. X-ray microanalysis of the mitochondria of the cold acclimated cells showed lower K and Cl, and higher Ca concentrations than those of the warm acclimated ones. The Ca of the reacclimated rat decreased to the level of the warm acclimated rat. The reconstituted membrane of the organelle was investigated by patch clamp to measure the voltage and-or H+ sensitive currents during cold acclimation. The experiments revealed the presence of a small and a relatively large conductance that correspond to the Cl-channel and the Ca2+dependent K+channel in the inner membrane of the organelle of the cells during cold acclimation.