Quantitative analysis of nm23 gene product in human cancers

应用免疫印迹技术结合半定量免疫印迹技术，用抗nm23蛋白的单抗(泛素-242)进行免疫组织化学定量和免疫组织化学染色，对人正常组织、肺癌组织、子宫内膜癌组织中nm23蛋白的总量、H1/H2亚型的相对比值(H2/H1)进行了研究。所有组织，包括癌组织，都含有在20.5kD中识别为H1的亚型和18kD中识别为H2的亚型。在正常组织中，nm23总蛋白在肝、肾和肾上腺组织中表达较高，在心脏和肌肉组织中表达较少。在所检查的所有组织中，H2水平均高于H1水平，并且其H2/H1比率因组织而异。免疫组织化学显示实质细胞在肝、胰腺、肾上腺组织中染色均匀，在胃粘膜、肾组织中染色不均匀。另一方面，肺癌组织中H_1和H_2水平均高于非肿瘤性肺组织，而肺腺癌和鳞癌中肿瘤H_2/H_1比值低于非肿瘤性肺组织。我们的研究表明，肺腺癌组织中nm23蛋白的表达水平与肿瘤的组织分化程度显著相关，而与转移潜能和肿瘤分期无关。此外，在子宫内膜癌中，nm23-H1和H2的表达与临床分期、转移、肿瘤大小、肌层侵犯、雌激素受体及绝经无关。结果表明，nm23-H1和-H2的高表达与组织分化程度呈正相关。

The total amount of nm23 protein, relative rations of H1 and H2 isoform (H2/H1) in human normal tissues, lung cancer tissues, endometrial cancer tissues were studied in combination with semiquantitative immunoblot technique followed densitometry and immunohistochemistry using monoclonal antibody against nm23 protein (Pan-242). All tissues including cancer tissues contained both isoform recognized in 20.5 kD as H1 and 18kD as H2 isoforms. In normal tissues, the level of total nm23 protein was higher in liver, kidney and adrenal gland, and lesser in heart and muscle tissues. H2 levels were higher than H1 levels in all tissues, examined, and their H2/H1 rations were varied from tissue to tissue. The immunohistochemistry revealed that the parenchymal cells were homogeneously stained in liver, pancreas and adrenal gland tissues and heterogeneously stained in gastric mucosa and kidney tissues. On the other hand, in lung cancers, both H1 and H2 levels in cancer tissues were higher than non-neoplastic lung tissues, and tumor H2/H1 ratio were lower than non-neoplastic ones in adenocarcinoma and squamous cell carcinoma of the lung. Our study indicate that nm23 protein levels in adenocarcinoma of the lung were significantly correlated with histologic differentiation of tumors, whereas they were not correlated with metastasis potentials and tumor stage. Furthermore, in endometrial cancers, the expression of nm23-H1 and H2 was not correlated with stage, metastasis, tumor size, myometrial invasion, estrogen receptor or menopause. It follows from the findings presented that the high expression o nm23-H1 and -H2 is positively correlated with histological differentiation.