Origin, Distribution and Fine structure of the Sympathetic nerves in the rat TMJ.

采用共聚焦激光扫描显微镜，采用亲和生物素过氧化物酶法和间接免疫荧光技术，观察了完整大鼠和切除颈上神经节大鼠颞下颌关节（TMJ）内含有神经肽Y （NPY）、酪氨酸羟化酶（TH）和血管活性肠多肽（VIP）的神经纤维的分布。NPY-和th阳性纤维的神经支配模式与VIP阳性纤维不同，前者同时分布于浅亚层和深亚层，后者主要分布于深亚层。NPY-和th -免疫反应纤维主要局限于血管成分；关节腔附近滑膜层偶见纤维。NPY-和TH-LI的神经数量更多地靠近上关节室而不是靠近下关节室。在切除上消化道动物的TMJ中，NYP和th免疫反应性显著降低，表明这些神经的交感神经来源。这些多肽可能在生理和病理生理条件下参与血管的调节和调节功能。其次，为了解出生后（0-32 d）神经和滑膜分布的变化，研究神经分布与P物质(SP) -、降钙素基因相关肽(CGRP) -和NPY的免疫反应性。第3天滑膜上观察到SP-或cgrp -免疫反应纤维，第10天观察到npy免疫反应神经。因此，感觉SP-或CGRP-纤维的支配早于交感NPY纤维。

The distribution of nerve fibers containing neuropeptide Y (NPY), tyrosine hydroxylase (TH) and vasoactive intestinal polypeptide (VIP) in rat temporomandiblar joint (TMJ) was examined in intact rats and in rats subjected to removal of superior cervical ganglion by both avidinbiotin peroxidase procedure and indirect immunofluorescence technique using confocal laser scanning microscopy. The innervation pattern of NPY- and TH-positive fibers differed from that of VIP positive fibers, namely, former fibers distributed to both superficial and deep sublinig layr while latter mostly located deeper sublining layr. NPY- and TH-immunoreactive fibers were largely confined to vascular elements ; occasional fibers were observed in synovial lining layr close to joint cavity. Number of nerves with NPY- and TH-LI were more observed close to the upper joint compartment rather than to the lower compartment. NYP and TH-immunoreactivities were dramatically reduced in the TMJ of superior gaglionectomized animals, indicating the sympathetic origin of these nerves. These peptides might be involved in vascular regulatory and modulatory function in physiological and pathophysiological conditions.Second, to know the postnatal developmental (0-32 days) changes of the distribution of nerves and the synovial membrane, the distribution of nerves with the immunoreactivities of substance P (SP) -, calcitonin gene-related peptide (CGRP) -, and NPY was investigated. In the synovial membrane SP- or CGRP-immunoreactive fibers were observed at day 3, while NPY-immunoreactive nerves were observed at day10. Thus, sensory SP- or CGRP- fibers innervate earlier than the sympathetic NPY fibers.