Reconstruction of temporomandibular joint tissue with mesenchymal Cells from synovial membrane

滑膜间充质细胞重建颞下颌关节组织

• 批准号: 20390514
• 负责人: FUJIMURA Kazuma
• 金额: $ 11.73万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20390514/
• 关键词: 滑膜; 顎関節; 滑液; 滑膜細胞; 顎関節症; 分化能

Mesenchymal stem cell are fasciating soureces for regenerative medicine. In previous studies, the ability of the proliferation and differentiation was different. Therefore, we investigated that this time clarify the conection between the clinical symptoms and the differentiation potential. Syonovial fluid was obtained from 13 patients with temporomandibular disorder (TMD). We culutured the adherent cells of the 6 in 13 samples that had the detail clinical findings. The subjects were divided into 2 groups. The first group included high differentiation of osteoblast and chondrocyte. The second group included poor differentiation.The clinic symptoms was measured using the pain score and the mouth-opening distance. The pain score was assessed using a visual analogue scale. There was a significant difference in the osteogenic and chondrogenic potential among the two groups. In the clinical findings, there was a significant correlation in the pain score among the two groups. However, no difference was observed the mouth-opening distance. These results were demonstrated that osteogenic and chondrogenic potential of synovium-derived cells were significantly different in TMD.

间充质干细胞是再生医学的重要来源。在以往的研究中，细胞的增殖和分化能力不同。因此，我们研究了这次澄清临床症状和分化潜能之间的联系。对13例颞下颌关节紊乱病(TMD)患者进行关节腔积液检查。我们对13个有详细临床表现的样本中的6个进行了贴壁细胞培养。受试者分为2组。第一组包括成骨细胞和软骨细胞的高分化。第二组为低分化组，临床症状用疼痛评分和张口距离来衡量。疼痛评分采用视觉模拟评分。两组间的成骨和成软骨潜能差异有统计学意义。在临床表现中，两组患者的疼痛评分有显著相关性。张口距离无明显差异。这些结果表明，在TMD中，滑膜来源的细胞的成骨和成软骨的能力有显著差异。

项目成果

顎関節患者における滑液由来の骨芽細胞・軟骨細胞の分化能と臨床症状の関連について

颞下颌关节患者滑液源性成骨细胞和软骨细胞分化潜能与临床症状的关系

• 发表时间: 2009
• 作者: 藤村和磨;別所和久;他
• 通讯作者: 他

顎関節症患者における滑液由来細胞の骨芽細胞・軟骨細胞の分化能と臨床症状の関連について

颞下颌关节紊乱病患者滑液来源细胞成骨细胞/软骨细胞分化潜能与临床症状的关系

• 发表时间: 2009
• 作者: Seo K;Seino H;Yoshikawa H;Petrenko AB;馬場洋;藤原直士;Someya G;Kawano Y;Maeda T;松田将門;Kanematsu T;Hirata M.;大久保康則
• 通讯作者: 大久保康則