STUDY ON MECHANISM OF EPIDERMAL EXCORIARION CAUSED BY EXFOLIATIVE TOXIN,USING MOUSE EMBRYOS

材料和方法&gt；用光镜观察2日龄小鼠和6月龄小鼠(N26月龄)和胚胎13~18d(E13-E18)小鼠皮肤的阴离子部位。对E13-E18、E15和E18的皮肤标本进行电子显微镜观察。对E15和N6m进行以下前处理：a)未处理b)透明质酸酶c)肝素酶、软骨素酶ABC和角聚糖酶，并用聚乙烯亚胺(PEI)中和E15和N6m表皮内的全部阴离子位点。结果&gt；IET可诱导E14和E15的表皮形成基底层以上裂隙。随着颗粒层的形成，解理面从基底上移至颗粒层…更多。最后，N2-a的表皮内裂隙仅发生在颗粒层内，在E13和E14的相邻细胞之间发现了阴离子部位。E15~E18，除基底层外，其余各层表皮上的阴离子部位均被抛弃。随着皮肤的成熟，阴离子部位集中在颗粒层。E16-E18和N2的毛乳头细胞间隙中也有丰富的阴离子中心，表皮中的II-b阴离子中心变化不大，但经透明质酸酶处理后，毛乳头中的阴离子中心显著减少，两个区域的II-c阴离子中心均不受酶处理的影响。所有酶处理的小鼠表皮没有改变，但毛乳头的阴离子位置减少。III PEI灌流导致E15小鼠的表皮内基底层和N2的颗粒层内发生分裂。IV我们观察到铁胶体颗粒弥漫地分布在小鼠表皮所有层的细胞内和细胞间。在本研究中未发现特定的阴离子位点。但在毛乳头的细胞间隙中发现了特定的阴离子位点。较少

<Material and method>The skin samples of 2-day-old mice and 6-month-old mice (N2 and N6m), and mouse embryos on embryonic days 13 to 18 (E13-E18) were used for observation of anionic sites with light microscopy.The skin samples of N2, E15, and E18 were used for observation with electron microscopy.I Skin of E13-E18, and N2 were incubated with ET,and we observed localization of intraepidermal cleft.II Skin of E13-E18, N2, and N6m were stained with fine cationic cacodylate iron colloid (Fe-cac-f) after the following pre-treatments :a) non-treatment b) hyaluronidase c) heparitinase, chondroitinase ABC,and keratanase respectively, and all of themIII Anionic sites within the epidermis of E15 and N2 were neutralized with polyethyleneimine (PEI).IV Skin of E15, E18, and N2 were observed with EM after Fe-cac-f staining.<Results>IET induced suprabasal cleft formation in the epidermis of E14 and E15. As the granular layr formed, the cleavage plane shifted from the suprabasal to the granular layr … More . Finally intraepidermal clefts occured only within the granular layr in N2.II-a Anionic sites were found between adjacent cells in the epidermis of E13 and E14. On E15 to E18, anionic sites became abandant throughout all the layrs of the epidermis except the basal layr. As the skin matured, anionic sites concentrated in the granular layr. And anionic sites were also abundant in the intercellular spaces of hair papilla of E16-E18 and N2.II-b Anionic sites in the epidermis did not change so much, but those in the hair papilla were much decreased by hyaluronidase treatment.II-c Anionic sites of both regions were not altered by respective enzyme treatment. Treatment by all enzymes made no change in the epidermis, but decreased anionic sites of the hair papilla.III PEI perfusion resulted in intraepidermal splitting that occured within the suprabasal layr in the epidermis of E15, and within the granular layr in the epidermis of N2.IV We observed that Fe colloidal particles were distributed diffusely in intra- and intercellular spaces of any cells of all layrs in the epidermis of mice. Specific anionic sites were not found in this study. But specific anionic sites were found in intercellular spaces of hair pappila. Less