Studies on the pathogenesis of cutaneous infections with S.aureus

1）应用免疫组化和免疫电镜技术观察纤维连接蛋白在金黄色葡萄球菌感染中的作用。可溶性纤连蛋白开始存款在真皮中的细颗粒图案，并逐渐增加，随着时间的推移。在24小时，两种模式的结合。在一种模式中，纤连蛋白结合到微生物的细胞表面的一部分。这表明纤连蛋白帮助胰岛细胞附着在纤维蛋白或结缔组织上。纤连蛋白结合的另一种模式是细胞周围的纤连蛋白结合。2）通过表皮接种金黄色葡萄球菌建立小鼠金黄色葡萄球菌性脓疱病模型，光镜和电镜观察其病变情况。本研究建立的人脓疱疮样水疱模型有助于揭示金黄色葡萄球菌在表皮形成水疱的机制。电子显微镜 ...更多信息 金黄色葡萄球菌在1/4 h和1h时侵入角质层，在金黄色葡萄球菌和角质层之间可见明显的晕圈。金黄色葡萄球菌细胞通过纤维样结构附着于周围角质细胞。在后来的时间点没有观察到这些结构。这一过程被认为是建立感染的必要的第一步。3）在评价局部或全身抗菌药物治疗脓疱病的过程中，通过表皮接种从人脓疱病中分离的菌株，在小鼠中产生葡萄球菌脓疱病。虽然经过充分的化疗后，病变中的金黄色葡萄球菌细胞形态发生了变化，但活的金黄色葡萄球菌细胞仍保留在角质层中。这可能表明角质层中的微生物不容易被抗菌剂根除。4）在使用小鼠的实验性脓毒症模型中，我们观察到金黄色葡萄球菌不能附着在正常皮肤上，但可以容易地附着并在受损皮肤和/或巴豆油刺激的皮肤上增殖。少

1)We observed the role of fibronectin in S.aureus infection using immunohistochemistry and immunoelectron microscopy. Soluble fibronectin began to deposit in a fine granular pattern in the dermis and gradually increased with time. At 24 h two patterns of binding were seen. In one pattern, fibronectin was bound to a part of the cell surface of microorganisms. This suggests that fibronectin helped staphylococcus cells to attach to fibrin or connective tissues. The other pattern of fibronectin binding was the binding of fibronectin all around the cells. The pattern could be interpreted as inhibition by fibronectin of S.aureus attachment to the fibrins and tissues.2)We produced an experimental staphylococcal impetigo model in mice by epicutaneous inoculation with S.aureus and observed the lesions by microscopy and electronmicroscopy. Our new method produce human impetigo-like blister may contribute to disclosing the mechanisms of blister formation in epidermis by S.aureus. Electronmicrosco … More pically, S.aureus cells invaded the horny layer at 1/4 h and 1 h, and a clear halo was seen between S.aureus cells and horny cells. S.aureus cells attached to surrounding horny cells by fibril-like structures. These structures were not observed at later points in time. This process is thought to be a necessary first step for the establishment of the infection.3)Staphylococcal impetigo was produced in mice by epicutaneous inoculation of a strain isolated from human impetigo in the evaluation of topical or systemic antimicrobial agents in the treatment of impetigo. Although after a sufficient chemotherapy S.aureus cells in the lesions morphologically changed, viable S.aureus cells remain in the horny layer. It could suggest that microorganisms in the horny layer are not easily eradicated by antimicrobial agents.4)In the experimental sepsis model using mice, we observed that S.aureus could not attach to the normal skin but could attach easily and proliferated on the injured skin and/or irritated skin with croton oil. Less