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Studies on the pathogenesis of cutaneous infections with S.aureus

金黄色葡萄球菌皮肤感染发病机制的研究

基本信息

批准号：
02670478
负责人：
ARATA Jiro
金额：
$ 1.41万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1990
资助国家：
日本
起止时间：
1990 至 1992
项目状态：
已结题

项目摘要

1)We observed the role of fibronectin in S.aureus infection using immunohistochemistry and immunoelectron microscopy. Soluble fibronectin began to deposit in a fine granular pattern in the dermis and gradually increased with time. At 24 h two patterns of binding were seen. In one pattern, fibronectin was bound to a part of the cell surface of microorganisms. This suggests that fibronectin helped staphylococcus cells to attach to fibrin or connective tissues. The other pattern of fibronectin binding was the binding of fibronectin all around the cells. The pattern could be interpreted as inhibition by fibronectin of S.aureus attachment to the fibrins and tissues.2)We produced an experimental staphylococcal impetigo model in mice by epicutaneous inoculation with S.aureus and observed the lesions by microscopy and electronmicroscopy. Our new method produce human impetigo-like blister may contribute to disclosing the mechanisms of blister formation in epidermis by S.aureus. Electronmicrosco … More pically, S.aureus cells invaded the horny layer at 1/4 h and 1 h, and a clear halo was seen between S.aureus cells and horny cells. S.aureus cells attached to surrounding horny cells by fibril-like structures. These structures were not observed at later points in time. This process is thought to be a necessary first step for the establishment of the infection.3)Staphylococcal impetigo was produced in mice by epicutaneous inoculation of a strain isolated from human impetigo in the evaluation of topical or systemic antimicrobial agents in the treatment of impetigo. Although after a sufficient chemotherapy S.aureus cells in the lesions morphologically changed, viable S.aureus cells remain in the horny layer. It could suggest that microorganisms in the horny layer are not easily eradicated by antimicrobial agents.4)In the experimental sepsis model using mice, we observed that S.aureus could not attach to the normal skin but could attach easily and proliferated on the injured skin and/or irritated skin with croton oil. Less

1)我们通过免疫组化和免疫电镜观察了纤连蛋白在金黄色葡萄球菌感染中的作用。可溶性纤连蛋白开始在真皮中以细颗粒状沉积，并随着时间的推移逐渐增加。 24小时时观察到两种结合模式。在一种模式中，纤连蛋白与微生物细胞表面的一部分结合。这表明纤连蛋白帮助葡萄球菌细胞附着在纤维蛋白或结缔组织上。纤连蛋白结合的另一种模式是纤连蛋白在细胞周围的结合。该模式可以解释为纤连蛋白抑制金黄色葡萄球菌附着在纤维蛋白和组织上。2)我们通过皮下接种金黄色葡萄球菌在小鼠中制作了实验性葡萄球菌脓疱疮模型，并通过显微镜和电子显微镜观察了病变情况。我们产生人类脓疱病样水疱的新方法可能有助于揭示金黄色葡萄球菌在表皮中形成水疱的机制。电镜观察，1/4 h和1 h时金黄色葡萄球菌细胞侵入角质层，金黄色葡萄球菌细胞与角质细胞之间可见清晰的晕圈。金黄色葡萄球菌细胞通过原纤维样结构附着在周围的角质细胞上。这些结构在后来的时间点上没有被观察到。这一过程被认为是建立感染所必需的第一步。3)在评价局部或全身抗菌药物治疗脓疱疮时，通过表皮接种从人脓疱疮中分离出的菌株，在小鼠中产生脓疱疮葡萄球菌。尽管经过充分的化疗后，病变中的金黄色葡萄球菌细胞形态发生了变化，但存活的金黄色葡萄球菌细胞仍保留在角质层中。这可能表明角质层中的微生物不容易被抗菌药物根除。4)在小鼠实验性脓毒症模型中，我们观察到金黄色葡萄球菌不能附着在正常皮肤上，但很容易附着在受伤的皮肤和/或巴豆油刺激的皮肤上并增殖。较少的