Implantation of human colorectal carcinoma cells in the liver and lymph nodes studied by in vivo fluorescence videomicroscopy

1. 活体荧光视频显微镜观察：人大肠癌细胞接种于裸鼠脾脏，捕获于肝窦1区。超过90%的肝癌细胞在注射后24小时内死亡。肝癌细胞在肝窦内表现出严重的畸形，这可能导致肝癌细胞在肝脏内死亡（clinin Exp Metastasis, 14,1996）。结直肠癌患者血液中游离癌细胞的遗传学诊断：采用RT-PCR技术检测结直肠癌患者血液中的游离癌细胞。这个结果是否能预测癌症术后复发，目前还在观察中。小鼠结肠26细胞的实验性肝转移：建立小鼠结肠26腺癌细胞的实验性肝转移。Colon26强烈表达CD44，抗CD44抗体抑制肝菌落的产生。CD44阳性人结直肠癌细胞的建立：将人CD44亚型CD44v3-10克隆并转染到CD44阴性结直肠癌细胞系中。建立的CD44v3-10阳性细胞系与透明质酸具有粘附性，而亲本细胞系没有。这种转化在裸鼠皮下植入后显示出生长延迟（外科论坛，1997,47）。

1. In vivo fluorescence videomicroscopoy : Human colorectal carcinoma cells inoculated into the spleens of nude mice were trapped at the zone 1 hepatic sinusoids. More than 90% of carcinoma cells died in the liver within 24 hr of injection. Carcinoma cells showed severe deformity in the hepatic sinusoids, which may have caused cancer cell death in the liver (Clin Exp Metastasis, 14,1996).2. Genetic diagnosis of free cancer cells in the blood of patients with colorectal cancer : Free cancer cells were detected in the blood of patients with colorectal cancer by the RT-PCR technique. Whether or not this result can predict postoperative recurrence of cancer is now under observation.3. Experimental hepatic metastasis with murine colon26 cells : Experimental hepatic metastasis was established with the murine colon26 adenocarcinoma cells. Colon26 strongly expressed CD44 and an anti-CD44 antibody inhibited production of liver colonies.4. Establishment of CD44-positive human colorectal carcinoma cells : A human CD44 isoform, CD44v3-10, was cloned and transfected into a CD44-negative colorectal cancer cell line. The established CD44v3-10 -positive line adhered to hyaluronate while the parental line did not. The transformant showed delay in growth when implanted subcutaneously in nude mice (Surgical Forum, 47,1996).