Study on the Carcinogenesis and the Morphogenic Factors of Gallbladder Cancer.

为了研究调控胆囊癌生长和形态的因素，我们在三维胶原凝胶中检测了正常胆囊腺癌细胞和胆囊癌细胞系的生长和形态。原代正常胆囊腺癌细胞形成囊状结构，加入EGF、HGF或表吗啡后，囊状结构增强，但作用程度不同。相反，传代的细胞未形成囊状结构，但加入成纤维细胞条件培养液或与成纤维细胞共培养使其囊化。提示成纤维细胞分泌一定的促进囊变的因子(S)。另一方面，胆囊癌细胞株很少在胶原凝胶中出现囊状结构，上述因素不促进囊变的形成。只有gb-d1形成囊状结构，尤其是在漂浮的胶原胶中。EGF、HGF和成纤维细胞条件培养液有刺激分枝或管状形态发生的倾向，上述差异可能是由于细胞内信号转导或某些黏附分子表达的差异所致。

To study the factors which regulate growth and morphology of gallbladder cancer, we have examined those of normal gallbladder cell and cell lines of gallbladder cancer in three-dimensioned collagen gels.Primary normal gallbladder cell formed cystic structure, which was enhanced by addition of EGF,HGF or epimorphin, whereas there were variations in the degree of the effects. On the contrary the subcultued cells had not formed cystic structure, but the addition of conditioned medium of fibroblasts or coculture with fibroblasts made them cystic. It was suggested that fibroblasts secrete some factor(s) which promotes regain of the cystic morphogenesis.On the other hand, gallbladder cancer cell lines rarely show cystic structure in collagen gels, and the above factors did not promote the cystic formation. Only GB-d1 formed cystic structure especially in floating collagen gel. EGF,HGF and conditioned medium of fibroblasts had the tendencies to stimulate the morphogenesis for branching or tubular formation.The above-mentioned differences between the normal and cancer cells supposed to be caused by the differences of intracellular signal transduction or those of the expressions of some adhesion molecules.