The Influence of Amrinone on the Oxygen Affinity of Red Blood Cell

我们研究了氨利酮对红细胞氧亲和力的影响。红细胞经克雷布斯-林格液离心后分为四组。（对照组，A1：加入临床使用浓度的氨酮，A2组：10倍于A1， A3组：100倍于A1）在37 C中孵育3小时后，测定P50、2,3- dpg、pH-e、pH-i、PCO2、碱过量、血糖、Na-e、Na-i、K-e、K-i。P50无变化，分别为24.1、24.6、25.1、25.8（对照组、A3、A2、A1）。与对照组相比，A3组和A2组2,3- dpg、pH-e和碱基过剩显著降低。与对照组相比，A3组的pH-i、PCO2和血糖均明显降低。我们得出结论，尽管氨利酮加速了红细胞的代谢，但它并没有降低红细胞的氧亲和力。

We investigated the influence of amrinone on the oxygen affinity of red blood cell. After centrifuge, red blood cell with Krebs-Ringer solution was divided into four groups. (Control Group, A1 : added to the amrinone of the concentration for the clinical use, Group A2 : ten times of the A1, Group A3 : one hundred times of the A1) After incubation in 37ﾟC for three hours, we measured P50,2,3-DPG,pH-e, pH-i, PCO2, base excess, blood sugar, Na-e, Na-i, K-e, K-i.P50 was not changed as 24.1,24.6,25.1,25.8 (Control, A3, A2, A1). But 2,3-DPG,pH-e, and base excess were decreased significantly in group A3 and A2 versus control group. Furthermore pH-i, PCO2 and blood sugar were decreased significantly in group A3 vs.control group.We concluded that amrinone did not decreased the oxygen affinity of red blood cell in spite of the acceleration of the metabolism.