Urine Protein profile System for Pathophysiologic Diagnosis of Renal and Urogenital Disorders

本项目已按计划顺利完成。A)重组蛋白1的制备及其抑制磷脂酶A2和磷脂酶C的生物活性研究目前正在进行表位分析。B)蛋白1在前列腺和女性前列腺上皮细胞中的免疫组织化学染色。C)证实了吸烟者血清蛋白1水平降低的机制是由于肺Clara细胞数量减少及其合成减少所致。D)建立了测定尿α2-巨球蛋白的乳胶凝集光度分析方法。E)对尿碱性胎儿蛋白(BFP)的乳胶凝集试验进行评价，并在精液中发现该蛋白在结构上与天然BFP相同。f)建立BFP作为非特异性炎症和肿瘤标记物的尿液标志物。G)对一种新的国际血浆蛋白标准物质进行评估，为今后评估一种新的尿蛋白标准物质奠定了基础。H)尿PSA的研究--其检测方法的评价和临床应用。在这些研究工作的基础上，正在构建尿蛋白图谱系统及其专家系统，以完成每种关键蛋白质的参考区间。该系统最迟将在一年内启动临床试验。

The present project was successfully finished as planned before. During two years, the following research have been accomplished or are underway which are summarized as followed : a) Preparation of recombinant protein 1 and demonstration of its biological activity on inhibitory effects on phospholipase A2 and phospholipase C.Epitope analyzes are now under way. b) Immunohistochemical demonstration of protein 1 in epithelial cells of prostate and female prostate designated as Skene's gland. c) Demonstration of mechanisms of decreased serum protein 1 value in smokers due to decreased number of lung Clara cells and its decreased synthesis. d) Development of a latex agglutination photometric assay for urinary alpha2-macroglobulin. e) Evaluation of an Latex agglutination test for urinary basic fetoprotein (BFP) and discovery of the protein in semen structurally identical with native BFP.f) Establishment of BFP as a urinary marker for nonspecific inflammatory and tumor marker. g) Evaluation of a new international reference material for plasma proteins which gave the basis for future evaluation of a new reference material for urinary proteins. h) A study on urinary PSA-its evaluation of an assay and clinical application.Based on these research works, urine protein profiles systems are being constructed with completion of reference intervals for each key proteins, and its expert system. Clinical trials by the system will be initiated within a year at latest.