Neurogenesis and organogenesis of zebrafish and medaka

Mishina m(原理图。东京医科大学的研究人员发现，三甲基补骨脂素（TMP）能高效地产生斑马鱼突变体，并分析了一种能增强感觉神经元分支的edawakare基因。Wakamatsu， Y.（名古屋大学生物科学中心）将胚胎细胞的细胞核移植到有细胞核的未受精卵上，产生了核移植的Medaka，发现携带donar基因的胚胎体形成率为32%，正常发育率为4%。Nakayasu， H.（生物学，冈山大学）利用免疫组织化学标记建立了斑马鱼大脑的外植体培养。脑科学。Islet-2通过过表达LIM/同源结构域蛋白来控制胚胎斑马鱼感觉神经元和运动神经元的轴向寻路。杨爱瑾,n(地中海。大阪大学（Sch. Osaka university） 9分离出一种新的zfh家族转录因子，发现其通过过表达参与背腹轴的决定。上野,n (Nat。本月,基本。Biol.)发现BMP参与外胚层图案的形成；它诱导腹侧表皮，并抑制神经发育。武田,h(杂志。科学。名古屋大学（Nagoya university）发现斑马鱼的神经诱导有两条途径：一条是结合BMP的组织者因子，另一条是参与后神经诱导并与BMP竞争的FGF。Yamagata， K.（东京都市神经科学研究所）发现了一种新的即时早期基因，即由LTP或电击诱导的arcadlin基因。它与钙粘蛋白同源，具有细胞粘附活性，提示与突触可塑性有关。

Mishina, M.(Sch. Med. Univ. Tokyo) found that trimethylpsoralen (TMP) produced zebrafish mutants in high efficiency and analyzed a edawakare gene which produces enhancement of branching of sensory neurons.Wakamatsu, Y.(Biosci Center, Nagoya Univ.) produced nuclear-transplanted Medaka by transplantation of nuclei from embryonic cells to unfertilized eggs with nuclei and found that formation of the embryonic body with donar gene was 32% and 4% of them normally developed.Nakayasu, H.(Biology, Okayama Univ.) established the explant culture of zebrafish brains by using immunohistochemical markers.Okamoto, H.(Brain sci. Inst. RIKEN) found that Islet-2 control axaonal pathfinding of both sensory and motor neurons in embryonic zebrafish by overexpressing LIM/homeodomain protein.Miki, N.(Med. Sch. Osaka Univ.9 isolated a novel zfh family transcription factor and found that it is involved in the determination of dorsoventral axis by overexpressing it.Ueno, N.(Nat. Inst. Basic. Biol.) found that BMP is involved in the ectodermal pattern formation ; it induces the epidermis in the ventral side and also suppresses the neural development.Takeda, H.(Biol. Sci. Nagoya Univ.) found that two pathways are involved in the neural induction in the zebrafish : one is organizer factors which binds to BMP, and the other is FGF which is involved in postrior neural induction and competes with BMP.Yamagata, K.(Tokyo Metropolitan Inst. Neurosci.) found a novel immediate early gene, arcadlin which is induced by LTP or electric shock. It has a homology with cadherin and has a cell adhesion activity, suggesting the involvement of synaptic plasticity.