Study on Anti-Cerebral Mararia Substances

1)通过原子显微镜（ARM）检查寄生虫感染的红细胞（iRBC）。ARM揭示了旋钮，恶性疟原虫或牛巴氏杆菌红细胞的表面突起，有一个正电荷，其余的都带负电荷。这些结果表明，球形突起的正电荷在iRBC与负电荷毛细血管上皮细胞和红细胞的粘附过程中起重要作用。一个iRBC粘附到几个RBC上，重新形成玫瑰花结。唾液酸的组成成分N-乙酰神经氨酸可抑制红细胞与C32细胞的粘附。3）AFM观察到恶性疟原虫、弓形虫、亚马逊利什曼原虫、克氏锥虫等侵入性原虫的接触部位都带正电荷，且在细胞表面形成了一个带正电荷的接触部位。4）泰国曼谷热带病医院采用青蒿琥酯联合去铁胺或喷替福林治疗重症疟疾患者。青蒿琥酯和去铁胺之间没有拮抗作用，这些试剂的联合给药加速了肝细胞功能障碍的恢复。青蒿琥酯与戊巴比妥钠联合用药的效果与青蒿琥酯无差异。

1) Parasites infected erythrocytes (iRBCs) were examined by atomic farce microscopy (ARM). ARM revealed knobs, surface protrusions of Plasmodium falciparum or Babesia bovis-RBCs, have a positive charge and that the rest were negatively charged..These results suggested that the positive charge of the knob plays an important role on the adhesion process of these iRBCs to negatively charged epithelluim cells of capillaries and erythiocytes. An iRBC adheres to several RBCs, reselting in rosette formation. The rosette formation process was inhibited by N-acetyl neuramic acid, a constitute of sialic acid.2) Suramin and heparin inhibited adhesion of P.faalciparizm-iRBCs to C32 cells. ARM observations of P.falcipa.rum-iIlBCs treated with these reagents suggest that the inhibition was ascribed to neutrarization of the positive charge of the knob by the poly-anionic reagents.3) AFM revealed that invasive forms of protozoan parasites (P.falciparum, Toxoplasma gondii, Leishmania amazonesis, Trypanosoma cruzz) have positively charged contact sit.. to their hostcells.4) Combination of artesunate and deferoxamine or pentoxifylline were used for the treatment of patients with severe malaria at Bangkok Hospital for Tropical Desieases in Thailand. No antagonism between artesunate and deferoxamine was found and co-administration of these reagents hastened recovery from hepatocellular dysfunction. Effects of combination of artrsunate and pentoxifyiline were not different from these of artesuanate.