Development of ESR endoscope detecting free radicals formed in the human body.

我们已经开发出ESR内窥镜的原型，它能够获得人体内产生的自由基的信息。电子自旋共振内窥镜是将电子自旋共振波谱和内窥镜相结合设计的，既能获得可视化信息，又能获得自由基分布信息。电子自旋共振内窥镜是L波段电子自旋共振波谱仪，采用单圈线圈，工作在700 MHz~1.5 GHz的低微波频率下，除了能测量自由基的分布外，还能测量任意自由基的电子自旋共振波谱。采用包括最新研制的细同轴电缆在内的现代技术，研制了适用于普通内窥镜(直径3 mm)、直径为2 mm的信号回转线圈的ESR探头内窥镜。该样机可作为人体普通内窥镜使用，还研制了用于ESR内窥镜的多功能环状线圈，可检测氧浓度，捕获自由基，寿命短。为了利用电子自旋共振内窥镜测量氧浓度，研制了覆盖透氧材料的环状线圈。此外，为了通过环路线圈捕获短寿命的自由基，我们还在环路线圈上覆盖了以自旋陷阱基团为侧链的聚合物材料。

We have developed the prototype of the ESR endoscope which is capable of getting information on free radicals generated in the human body. The ESR endoscope is designed as combing ESR spectroscopy and endoscope, thus it can get both the visual information and free radical distribution information. The ESR endoscope is the L-band ESR spectrometer with the single turn loop coil working at the low microwave frequency, 700 MHz to 1,5 GHz, and it can measure the ESR spectrum of any free radical species beside the distribution of the free radicals.During two years research period, we have focused on solving obstacles when combing the ESR spectroscopy and the endoscope, such as the interference of the CCD video signals with microwave signals. Using the modern technology including newly developed thin coaxial cables, we have mede the ESR endoscope with 2mm diameter signal turn loop coil for ESR probe, which is fit to the common endoscope (3 mm diameter). This prototype can be used as the general endoscope for human.We also developed the multi-functional loop coil used in the ESR endoscope, which can detect the oxygen concentration and trap free radicals with the short lifetime. To measure the oxygen concentration by the ESR endoscope, the loop coil covered with oxygen permeable materials was developed. Furthermore, to trap the short lifetime free radicals by the loop coil, we also made the loop coil covered with the polymer materials having spin trap goups as side chains.