Studies on Intensive Search of New Bioactive Marine Molecules and Their Supply for the Practical Uses

新的生物活性海洋分子的分离和结构鉴定及其用于药理用途的实际供应已经成功完成。品纳毒素，有毒的两性大气旋贝类属贝类，主要生活在印度洋和太平洋温带和热带的浅水区。本文报道了从冲绳松材线虫中分离得到的松茸毒素A、B、C和D的分离和结构测定。抑制血管细胞黏附分子-L诱导新生物碱Halichondria okadai中分离得到新生物碱Halichiorine，其结构经波谱确证。氟氯生显著抑制血管细胞黏附分子-1(VCAM-1)对人脐静脉内皮细胞的诱导作用。胞质磷脂酶A_2的抑制剂--品甲酸和牛磺酸从冲绳双壳贝类中分离得到两个新的具有CplA_2抑制活性的多酮类化合物--品甲酸和牛磺酸。去甲苯甲胺是一种很有前途的骨质疏松候选药物，它可以抑制去卵巢小鼠骨重量和强度的下降，可能是一种很好的骨质疏松药物。海洋生物膜形成的调控分子我们报道了抗生物膜化合物的分离和结构，如β-苯乙胺衍生物和Untenine A、B、C。

Isolation and structural elucidation of the novel bioactive marine molecules and their practical supply for pharmacological uses have been successfully performed.1. Pinnatoxins, Toxic Amphoteric MacrocyclesShellfish of the genus Pinna live mainly in shallow areas of the temperate and tropical zones of the Indian and Pacific Oceans. We reported the isolation and structure determination of pinnatoxins A, B, C and D from the Okinawan Pinna muricala.2. Halichlorine, an Inhibitor of VCAM-l InductionThe novel alkaloid halichiorine was isolated from Halichondria okadai and its structure was elucidated by spectroscopic analysis. Halichlorine significantly inhibits VCAM-1 (vascular cell adhesion molecule-1) induction in HUVEC (human umbilical vein endothelial cells).3. Pinnaic acid and Tauropinnaic acid, Inhibitors of Cytosolic PLA_2Two novel polyketides with cPLA_2 inhibitory activity, pinnaic acid and tauropinnaic acid were isolated from the Okinawan bivalve Pinna muricata.4. Norzoanthamine, a Promising Candidate for an Osteoporotic DrugNorzoanthamine hydrochloride, which suppresses the decrease in bone weight and strength in ovariectomized mice, could be a good candidate for an osteoporotic drug.5. Marine Molecules Regulating Biofilm FormationWe reproted the isolation and structure of anti-biofilm compounds such as beta-phenethylamine derivatives and untenines A, B, and C.