Application of ganglioside receptor for Theileria sergenti on the bovine erythrocytes to the prevention and treatment of bovine thelleriosis.

应用牛红细胞上的神经节苷脂受体预防和治疗牛肠杆菌病。将牛红细胞来源的i -活性神经节苷脂（NeuGc）插入明尼苏达沙门氏菌R595脂质体中，免疫BALB/c小鼠，获得了IgM类抗i -活性神经节苷脂（SHS-21）单克隆抗体。使用各种纯化的神经节苷脂来确定单克隆抗体的特异性。发现SHS-21抗体与i-活性神经节苷脂（NeuGc）和i-活性神经节苷脂（NeuGc）发生反应，但与其他所测神经节苷脂不发生反应。研究表明，牛红细胞表面的i -活性神经节苷脂（NeuGc）可能是瑟氏梭菌分裂子的附着侧。因此，SHS-21抗体被认为是治疗牛疯牛病的有效抗体。含i -活性神经节苷脂质体的制备。为了阐明是否能够制备具有i -活性神经节苷（NeuGc）的承载抗原虫剂的脂质体，进行了实验。实验结果表明，i -活性神经节苷脂（NeuGc）结合脂质体能够包封抗原虫剂。这种类型的脂质体可能导致新的途径，以治疗牛肠杆菌病。含i -活性神经节脂质体（NeuGc）在牛肠杆菌病疫苗研制中的应用。为了评价含有i -活性神经节苷脂（NeuGc）的脂质体作为口服牛乳杆菌病疫苗的有效性，研究了脂质体的稳定性和与脂质体相关的神经节苷抗原在口服后的抗体反应。由二棕榈酰磷脂酰胆碱（DPPC）、二棕榈酰磷脂酰丝氨酸（DPPS）和胆固醇（Chol）（1:1: 2，摩尔比）、二硬脂酰磷脂酰胆碱（DSPC）和Chol（7:2，摩尔比）、磷脂酰磷脂酰胆碱、DPPS和Chol（7:3:2或1:1:1:2，摩尔比）组成的脂质体在酸性溶液（pH 2.0）、胆汁和胰酶溶液中稳定。以DPPC、DPPS、Chol（摩尔比为1:1:2）组成的含有神经节苷脂抗原（神经节苷脂GM1）的脂质体口服免疫小鼠后，发现血清对神经节苷脂GM1产生IgA抗体应答。另一方面，这种脂质体不能通过口服诱导任何可检测到的抗神经节苷脂GM1 IgG和IgM抗体反应。未来，需要进一步的研究来开发脂质体，通过口服免疫产生血清IgG或IgM反应。少

Ganglioside receptor on the bovine erythrocytes was qpplied to the prevention and treatment of bovine theileriosis.1. Production of monocional antibody against gangliosiode receptor, 1-active ganglioside (NeuGc), on the bovine erythrocytes for T.sergentl.Monoclonal antibody against I-active ganglioside (NeuGc), SHS-21, of the IgM class was obtained by the immunization of BALB/c mice with bovine erythrocytes-derived I-active ganglioside (NeuGc) inserted into liposomes with Salmonella minnesota R595 lipopolysaccharides. Various purified gangliosides were used to determine the specificity of the monoclonal antibody. SHS-21 antibody was found to react with I-active ganglioside (NeuGc) and i-active ganglioside (NeuGc), but not with other gangliosides tested. It has been shown that I-active ganglioside (NeuGc) on the bovine erythrocytes surface was suggested to act as attachment side for T.sergenti merozoites. Thus, SHS-21 antibody is considered to be useful for the treatment of bovine theil … More eriosis.2. Preparation of drug-laden liposomes containing I-active ganglioside (NeuGc).To elucidate whether or not antiprotozoan agent-laden liposomes having I-active ganglioside (NeuGc) are able to prepare, experiment was performed. The result of the experiment was that I-active ganglioside (NeuGc) incorporated liposomes are able to encapsulate the antiprotozoan agent. This type of liposomes may lead to new approaches to the treatment of bovine theileriosis.3. Application of liposomes containing I-active gangliosiode (NeuGc) for development of bovine theileriosis vaccines.In order to evaluate the usefulness of liposomes containing I-active ganglioside (NeuGc) as oral vaccines for bovine theileriosis, the stability of liposomes and antibody responses to ganglioside antigen associated with liposomes after oral administration were examined. Liposomes composed of dipalmitoylphosphatidylcholine (DPPC), dipalmitoylphosphatidylserine (DPPS), and cholesterol (Chol) (1 : 1 : 2, molar ratio), distearoylphosphatidylchollne (DSPC) nd Chol (7 : 2, molar ratio), and DSPC,DPPS,and Chol (7 : 3 : 2 or 1 : 1 : 2, molar ratio) were stable in acidic solution (pH 2.0), bile, and pancreatin solution. After the oral immunization of ganglioside antigen (ganglioside GM1) -containing liposomes composed of DPPC,DPPS,and Chol (1 : 1 : 2, molar ratio) to mice, the serum IgA antibody responses against ganglioside GM1 were found. On the other hand, such liposomes were unable to induce any detectable anti-ganglioside GM1 IgG and IgM antibody responses by oral administration. In future, further studies will be necessary to develop the liposomes for the generation of serum IgG or IgM responses by oral immunization. Less