The effect of the central nervous system on apoptosis in the rat intestine

我们之前已经证明了中枢神经系统在大鼠肠粘膜细胞生长中起重要作用。本研究旨在探讨中枢神经系统除对肠黏膜细胞生长有影响外，是否对肠黏膜细胞凋亡有影响。细胞凋亡率用粘膜DNA碎片率测定。禁食24 h后，肠黏膜细胞凋亡增加。自由摄食大鼠的DNA片段率存在昼夜变化，在摄食后的黑暗时期下降。肠道细胞凋亡的昼夜变化与肠道鸟氨酸脱羧酶活性有镜像关系。大鼠第三脑室给药2-脱氧-d -葡萄糖有减少肠黏膜细胞凋亡的趋势。这种影响至少部分是通过外侧下丘脑产生的。这些结果提示中枢神经系统可能控制大鼠肠黏膜细胞凋亡。

We previously demonstrated that the central nervous system playd an important role in the cell growth of the rat intestinal mucosa. The aim of the present study was to examine if the central nervous system had any effect on apoptosis of the intestinal mucosa in addition to the cell growth. The rate of apoptosis was evaluated by the % fragmented DNA in the mucosa. After 24 h fasting, apoptosis increased in the intestinal mucosa. Percent fragmented DNA in the ad libitum feeding rat had a circadian variation which decreased in a dark period when the rat fed. The circadian variation of the intestinal apoptosis had a mirror image of the intestinal ornithine decarboxylase activity. Administration of 2-deoxy-D-glucose into the rat third cerebroventricle tended to decrease apoptosis in the rat intestinal mucosa. This effect was, at least in part, through the lateral hypothalamus. These results suggested the possibility that the central nervous system might control apoptosis in the rat intestinal mucosa.