Investigation for human papillomavirus type-specific cytopathic effect and related epidermoid cysts

1.在我们之前的研究中，我们发现hpv家族，HPV4,60和65，不仅与细胞质内包涵体的特定组织学特征有关，而且与色素疣的特征性临床表现有关。在这项研究中，我们已经清楚地证明黑色素颗粒在HPV /60/65相关疣中特异性增加，提示HPV型特异性色素紊乱（Br J Dermatol, 1998）。我们将在明年的项目中尝试免疫组织化学和分子生物学的研究来了解HPV型特异性色素紊乱的病理机制。HPV 60是唯一在足底表皮样囊肿中发现的病毒。在本研究中，我们在足底表皮样囊肿中发现了HPV 57，其组织学特征与HPV 60相关的足底表皮样囊肿不同（Br J Dermatol, 1998）。我们正在研究大量的足底表皮样囊肿与HPV 57之间的关系。我们利用计算机对hpv相关的足底表皮样囊肿的结构进行了三维重建分析，揭示了一个起源于内分泌管的表皮样囊肿的精细结构。

1.In our previous studies, it was revealed that a family of HPVs, HPV4,60 and 65, is associated with not only a specific histologic feature of the homnogeneous intracytoplasmic inclusion bodies but also a characteristic clinical picture of the pigmented warts. In this study we have clearly demonstrated that melanin granules were increased specifically in HPV4/60/65-associated warts, suggesting HPV type-specific pigmentary disorder (Br J Dermatol, 1998). We will attempt immunohistochemical and molecular biological studies to understand the pathomechanism of the HPV type-specific pigmentary disorder in our next year's project.2.HPV 60 is the only virus that has been found in plantar epidermoid cysts. In the present study we have found HPV 57 in a plantar epidermoid cyst which showed a different histological feature from that of HPV 60-associated plantar epidermoid cyst (Br J Dermatol, 1998). We are investigating the relationshop between the epidermoid cysts and HPV 57 in much larger number of plantar epidermoid cysts.Using computor we performed three-dimensional reconstruction analysis for the structure of HPV-associated plantar epidermoid cysts, revealing the fine structure of an epidermoid cyst of eccrine duct origin.