Anapylactic reaction and cytokine expression in gut form mice oral challnged with ovalbumin

我们得到了下面描述的结果。用卵清蛋白（OVA）和明矾腹腔免疫Balb/c小鼠，通过被动皮肤过敏反应（pCA）观察到较高的抗OVA IgE滴度.免疫组化结果显示，卵清蛋白可诱导肠黏膜固有层嗜酸性粒细胞浸润，6 h达高峰。预先给予抗小鼠IL-5单克隆抗体可显著抑制嗜酸性粒细胞浸润.原位杂交显示固有层内有IL-5 mRNA阳性细胞.逆转录聚合酶链反应（RT-PCR）检测肠道RANTES mRNA表达，但其表达并非非特异性。该方法未检测到Eotaxin mRNA.灌胃后6 h，肠组织中可见ICAM-1和VCAM-1 mRNA的瞬时表达，提示IL-5在嗜酸性粒细胞向肠组织浸润中起重要作用。

We got the results described below.1. Balb/c mice were intraperitonealy immunized with ovalbumin (OVA) and alum, and high anti-OVA IgE titers were observed by passive cutaneous anapylaxis (pCA).2. Antigen-specific significanti eosinophil infiltrations were observed in lamina propria of gut by oral challenge with OVA,and peaked at 6h.3. The eosinophil infiltrations were significantly inhibited by preadministration with monoclonal anti-mouse IL-5 antibody.4. IL-5 mRNA positive cells were demonstrated in lamina propria by in situ hybridization.5. RANTES mRNA expression was observed in gut by reverse transcription polymerase chain reaction (RT-PCR), but it was not non-specific. Eotaxin mRNA was not detected by the method.6. Transient mRNA expressions of ICAM-1 and VCAM-1 (6h after oral challenge) were observed in gut.These results suggested important role of IL-5 in eosinophil infiltration into gut.