Immunocytochemical and ultrastructural examination of amyloid plaques in the brains with Alzheimer-type dementia.

阿尔茨海默病脑内淀粉样斑块的详细免疫组织化学和超微结构检查。淀粉样斑块是老年斑的一种特殊类型，其中薄胶质束渗透到淀粉样原纤维团块中，伴随极少数轴突终末退化，Oyanagi（1990）首先在某些阿尔茨海默病病例的电子显微镜下描述了这一现象。研究对象：7例阿尔茨海默病临床及病理资料充分的病例。组织学研究。在两例中发现大量淀粉样斑块，其中淀粉样斑块倾向于发生在顶叶和颞叶皮层以及回底。3例患者仅在顶叶和颞叶的脑回底部发现少量淀粉样斑块。2例未见淀粉样斑块。免疫组织化学研究。淀粉样蛋白斑块的淀粉样蛋白核心对所有抗β -抗体均表现出阳性免疫反应。更多的淀粉样抗体识别1-40,1-42和8-17的氨基酸残基。在分子香料方面，鉴定出了Abeta1-40和Abeta1-42。抗gfap抗体染色渗透到淀粉样蛋白团块中的薄胶质束。抗tau抗体仅能观察到淀粉样蛋白团块周围的少数神经元突起。电子显微镜研究。在淀粉样斑块中发现星形细胞胶质束。在3例有淀粉样斑块的病例中发现了一种特殊形式的星形细胞变性，称为“星形细胞胶质缠结”。星形胶质缠结由类似phf的异常管状结构和神经原纤维缠结的直管状结构组成。galyas - braak浸渍银切片的免疫电镜和电镜观察。星形胶质细胞中的异常小管表现出嗜银性和抗tau免疫反应性。结论：淀粉样斑块是一种独特的老年斑类型，前者缺乏退化的轴突终末，前者对淀粉样斑块有强烈的星形细胞反应。没有发现淀粉样斑块的发生与临床持续时间之间的关系。少

This is a detailed immunohistochemical and ultrastructural examination of "amyloid plaques" in the brains of Alzheimer's disease. The amyloid plaque is a distinctive type of senile palques, in which thin glial bundles penetrate into the mass of amyloid fibrils accompanied with very few degenerating axon terminals, first described by Oyanagi (1990) under the electron microscope in certain cases of Alzheimer's disease.Subjects : Seven clinically and pathologically well-documented Alzheimer's disease cases were examinedResults : 1. Histological studies. Numerous amyloid plaques were found in two cases, in which amyloid plaques tended to occurred in the parietal and temporal cortices and in the floor of the gyrus. Only few amyloid plaques were found in the floor of gyri in the parietal and temporal lobes in three cases. No amyloid plaques were found in two cases.2. Immunohistochemical studies. The amyloid core of the amyloid plaques showed positive immunoreaction with all of the anti-beta- … More amyloid antibodies that recognized amino-acid residues of 1-40,1-42, and 8-17. In terms of molecular spices of Abeta-protein, both of Abeta1-40 and Abeta1-42 were identified. Anti-GFAP antibody stained thin glial bundles that penetrated into the amyloid mass. Anti-tau antibodies visualized only few neuronal processes surrounding the amyloid mass.3. Electron microscopic studies. Astrocytic glial bindles were identified in amyloid plaques. A particular form of astrocytic degeneration called "astrocytic glial tangles" were found in 3 cases that had amyloid plaques. The astrocytic glial tangles consisted of abnormal tubular structures that resembled PHFs and straight tubules of neurofibrillary tangles.4. Immuno-electron microscopy and electron microscopic observation of Gallyas-Braak's silver impregnation sections. Abnormal tubules in the astrocytes showed argyrophilia and anti-tau immunoreactivity.Conclusions : The amyloid plaque was a distinctive type of senile plaques in that the former lacked degenerating axon terminals and that the former had intense astrocytic reaction to the amyloid mass. Association between the occurrence of amyloid plaques and the clinical duration was not found. Less