The study of intrathymic islet transplantation.

本研究从三个方面探讨了同种异体胰岛移植后的免疫抑制作用：1.胸腺内移植胰岛的效果; 2.基因转染胰岛; 3.主要组织相容性复合物在猪胰岛移植中的作用。1.采用大鼠胰岛移植模型，移植到胸腺内的胰岛在抗淋巴细胞球蛋白的作用下存活100天以上。相比之下，移植到肾包膜下的胰岛存活31.5天（中位存活）。胸腺内移植胰岛可诱导供者特异性耐受。2.用脂质体法将pZeoSV 2/lacZ载体转染大鼠胰岛，经X-gal染色，转染率达90%以上，静态培养法检测胰岛内分泌功能良好。3.采用MHC（SLA）匹配的NIH小型猪，在无免疫抑制的情况下进行同种异体胰岛移植。受体猪表现出正常的免疫力超过两周。提示MHC在胰岛移植中的重要作用。结果表明，多种途径均可实现无持续免疫抑制的胰岛移植。

For the purpose of immunosuppression after pancreatic islet alloranft, the following three projects were studied ; 1.Efficacy of intrathymic islet alloranft, 2.Gene transfection into isolated islets, 3.The role of Major histocompatibility complex in porcine islet allograft. 1.Using rat islet allograft model, the islets transplanted into the thymus survived more than 100 days with a single antilymphocyte gloublin. In contrast, the islets transplanted beneith the kidney capsel survived 31.5 days (median survival). Furthermore, intrathymic islet allograft induced the donor-specific torelance evaluated by in vitro assay (MLR). 2.The islets isolated from Rat pancreata were transfected with pZeoSV2/lacZ Vector using a liposomal method Transfer rates evaluated by X-gal staining showed over 90% with good endocrine function assayd by static incubation. 3.Using a MHC (SLA) matched NIH miniature pigs, the islet allograft without immunosuppression was perfomed. The recipient pigs showed normoglycemia more than two weeks. This result suggested the important role of MHC on islet allograft. These results demonstrated that various approach might realize the islet allograft wothout continuous immnosuppression.