The prognosic value of the transcription of tumor growth-related genes in endoscopic biopsy specimens from patients with malignancies of digestive tracts

胃癌患者术前内镜检查时获得肿瘤活检标本。从每个活检标本中提取总RNA，并使用逆转录酶-聚合酶链反应分析（RT-PCR）来分析肿瘤生长相关基因的转录。细胞周期相关因子包括细胞周期蛋白D 1和细胞周期蛋白E，侵袭相关因子包括尿激酶型纤溶酶原激活剂和72 kd IV型胶原酶，血管生成相关因子包括血管内皮生长因子和血小板源性生长因子A，免疫抑制相关因子包括转化生长因子B b和白细胞介素10。（1）PDGF-A和TGF-β mRNA的表达分别是早期胃癌和进展期胃癌术前淋巴结转移的指标。(2)PDGF-A和TGF-b是术前预后指标。(3)术前评估每种癌的恶性潜能可能会导致更合理的胃癌分类。

The tumor biopsy specimens were obtained during preoperative endoscopy of patients with gastric carcinoma. The total RNA from each biopsy specimen was isolated and a reverse trascriptase-polymerase chain reaction analysis (RT-PCR) was used to analyze the transcription of tumor growth-related genes. The factors examined included cyclin D1 and cyclin E as cell cycle-related factors, urokinase-type plasminogen activator and 72-kd type IV collagenase as invasion-related ones, vascular endothelial growth factor and platelet-derived growth factor-A as angiogenesis-related ones, and transforming growth factor b and interleukin-10 as immunosuppression-related ones.Results :(1) The incidence of PDGF-A and TGF-b mRNA expression was preoperative indicator of lymph node involvement in early and advanced gastric cartcinomas, respctively.(2) PDGF-A and TGF-b were preoperative prognostic indicators.(3) Preoperative evaluation of malignant potential of each carcinoma may lead to a more reasonable classification for gastric carcinomas.