INVESTIGATION FOR IMMUNOGENETIC BACKGROUND OF ANTISPERM ANTIBODY,INVOLVEMENT OF HLA-II

应用聚合酶链式反应-SSO、聚合酶链式反应-单链构象多态和聚合酶链式反应-限制性片段长度多态性技术对36例血清精子结合抗体阳性的女性进行了人类白细胞抗原DR和DQ基因分型，并与日本正常人群(986个单倍型，1068个单倍型和2432个单倍型)进行了比较。HLADRB1和HLADQB1基因座的表型显著增加，而HLADQA1基因座的表型增加不明显。HLADRB1**0901基因频率为25%，正常人群为13.6%(P&lt；0.0085)；HLADRB1**1501患者为12.5%，正常人群为6.7%(P&lt；0.0025)；人类白细胞抗原-DRB1**0901-DQB1**0303单倍型在具有精子固定抗体的不育妇女中表现出最强的不平衡。HLADRB1**0901-DQB1**0303的这种单倍型在高加索人中非常罕见(从单倍型频率的0到几个百分点)，但在日本人中很高(约13%)。与其他国家的报道相比，日本患者中高频率的HLADRB1**0901-DQB1**0303可能是导致精子固定抗体出现频率较高的原因。

Thirty six women, possessing of sperm immobilizing antibody in their sera were examined of their HLA-DR and DQ types using DNA obtained from peripheral blood white cells.The typing of HLA-DR and DQ locus were performed by PCR-SSO,PCR-SSOP and PCR-RFLP.The determined haplotype frequencies of patients were estimated of statistical significance, compared to normal Japanese populations (986 haplotype for HLA-DRB1,1068 for HLA-DQA1 and 2432 for HLA-DQB1). There were significant increase in phenotypes of HLA-DRB1 and HLA-DQB1 locus but not in HLA-DQA1 locus. There were 25 gene frequency percent for HLA-DRB1**0901 in contrast to 13.6 percent in normal population (p<0.0085) , 12.5 percent of patients of HLA-DRB1**1501 in contrast to 6.7 percent of normal population (p<0.0025) , 13.9 percent in contrast to 6.8 percent of HLA-DQB1**0602 (p<0.0089) and 25 percent of patients in contrast to 14.8 percent of normal population of HLA-DQB1**0303 (p<0.0018). The most strong disequilibrium found in HLA-DRB1**0901-DQB1**0303 haplotype among infertile women possessing sperm immobilizing antibody. This haplotype of HLA-DRB1**0901-DQB1**0303 is very rare in Caucasians (from 0 to several percent of haplotype frequency) but characteristic high in Japanese (about 13 percent of that). The high frequency of HLA-DRB1**0901-DQB1**0303 among the patients may account for higher frequency of sperm immobilizing antibody in Japan compared with another country reports.It was elucidated that anti-sperm immunity of human was restricted and controlled by HLA-DR or DQ genes.