Cell adhesion molecules and cytokines in periodontal disease

我们研究了细胞粘附分子和细胞因子在牙周病中的作用，并得到以下结果。细胞和基质表征的主要差异在于成纤维细胞和细胞外基质之间的粘附。CD 43和约45-kDa的糖蛋白在成纤维细胞和免疫活性细胞之间的粘附序列中发挥作用。在LPS刺激下，增生牙龈成纤维细胞产生大量TGF-β和IL-6。在大鼠实验性牙周炎中，白细胞趋化因子不仅由炎性细胞产生，也由连接上皮细胞产生。龈沟液中肝细胞生长因子的含量与牙周病的其他参数如探诊深度、牙龈指数和IL-1β的含量呈正相关。在成纤维细胞上表达的几种PGE 2受体中，EP 2和EP 4受体被鉴定为与成纤维细胞增殖有关的受体。 ...更多信息 ICAM-1的表达。HSP 60刺激晚期牙周炎患者外周血单个核细胞后，IFN-γ在mRNA和蛋白水平的表达均显著增加。牙周病牙龈组织中单个核细胞分泌的IFN-γ与患者外周血单个核细胞分泌的IFN-γ的比值较低，而IL-4的比值较高。牙周炎患者牙龈淋巴细胞内的凋亡中断是由于信号传递减弱和分子凋亡抑制所致。伴放线菌免疫大鼠腹腔再感染后，TNF-α和IL-6的产生均增加，IFN-γ的产生也明显增加。从遗传学上鉴定了伴放线菌的五种血清型a至e。PCR技术和碱基序列分析导致血清型鉴定的方法，是非常有用的了解牙周病的发病机制和诊断。少

We studied the role of cell adhesion molecules and cytokines in periodontal diseases, and obtained the following results. The primary difference in the characterization of both cells and matrix was in the adhesion between fibroblasts and extracellular matrix. Both CD43 and a glycoprotein of about 45-kDa played roles in the adhesion sequence between fibroblasts and immunocompetent cells. Fibroblasts derived from hyperplastic gingiva produced abundant quantities of both TGF-β and IL-6 when stimulated by LPS.In rat experimental periodontitis, leukocyte chemotactic factor was produced not only by inflammatory cells but also by junctional epithelial cells. There was a direct correlation between the amount of hepatocyte growth factor in gingival crevicular fluid and other parameters of periodontal disease such as probing depth, gingival index and amount of IL-1β. Among the several PGE2 receptors expressed on fibroblasts, EP2 and EP4 receptors were identified as those involved with the inhibi … More tion of ICAM-1 expression. Stimulation by HSP60 of mononuclear cells from peripheral blood of patients with advanced periodontitis produced significantly increased expression of IFN-γ at both the mRNA and protein levels. The ratio of IFN-γ derived from mononuclear cells within periodontally involved gingival tissues to that of mononuclear cells from peripheral blood of the same patients was lower, whereas the respective ratio for IL -4 was higher. There was interruption of apoptosis within lymp hocytes from gingiva involved with periodontitis due to diminished signal transfer as well as molecular apoptosis inhibition. Peritoneal re-infection of rats immunized to A.actinomycetemcomitans resulted in increased production of both TNF-α and IL-6, thus leading to markedly increased production of IFN-γ. Five serotypes, a through e, of A.actinomycetemcomi tans were genetically identified. PCR techniques and base sequence analysis have led to methods of serotype identification that are very useful in understanding the pathogenesis and diagnosis of periodontal disease. Less