The establishment of the simple confirmation method of the flower-bud initiation in strawberry using the phenomenon of pectin accumulation in crown.

在草莓花芽萌发过程中，发现了草莓树冠内果胶积累的现象。本研究的目的是利用这一现象，建立一种简便的草莓花芽分化鉴定方法。首先，我们试图用从树冠中提取的果胶制造抗体。然而，来自小鼠的抗体敏感性低，不切实际。于是，我们研究了通过直接测定果胶来确定花芽分化的方法。本试验选用了4个品种。在显微镜下确定花芽萌发的阶段后，用FAA固定树冠。直到花芽发育从分化期进入雌蕊分化早期，果胶都大量积累到树冠上。FAA固定后第2天果胶形成凝胶状沉积，分化期果胶积累量为5 mg/500 mg树冠。将500 mg的牙冠固定在FAA 10ml中两天后，果胶的凝胶形态清晰可见。本研究未能建立利用抗原抗体反应确定花芽分化的方法。然而，通过FAA确认花芽起始时果胶在树冠中的积累，可以诊断花芽起始的开始。

We discovered the phenomenon that pectin accumulates in the strawberry crown according to the flower-bud initiation. Purpose of this research is to develop the easy confirmation method of the flower-bud initiation in strawberry by utilizing this phenomenon. First, we tried to make an antibody by using the pectin that extracted from the crown. However, the antibody from a mouse the was low sensitive, and not practical. Thereupon, we examined the method that flower-bud initiation is confirmed by measuring direct pectin. Four cultivars were used in the experiment. The crown was fixed with FAA after it was determined the stage of flower-bud initiation with a microscope. Until the development of flower-bud reaches to Early stage of pistil differentiation from Differentiated stage, the pectin was accumulated to the crown fairly plentifully in either cultivar. Pectin became a gel form deposit to the 2nd day after fixation with FAA.The accumulation quantity of the pectin in Differentiated stage is 5 mg to every 500 mg of crowns. Two days after 500 mg of the crowns were fixed to FAA 10 ml, the gel form pectin is confirmed clearly. It was not possible to establish the confirmation method of the flower-bud initiation by the antigen antibody reaction in this research. However, the start of flower-bud initiation is able to be diagnosed by confirming the accumulation of the pectin in the crown with flower-bud initiation by using FAA.