Inheritance of morphological and physiological characters in the inter-subspecific cross between Samia cynthia ricini and S. c. pryeri.

F1杂交种是由Samia cynthia pryeri♀与Samia cynthia ricini♂杂交而成。F1雌蛹未进入滞育，而大部分F1雄蛹进入滞育，说明蛹滞育特性是通过性别连锁的方式遗传的。在回交后代中，蛹滞育似乎与性别有关。在F1代和B1代中，蛹在较长的日照周期内未出现滞育现象。分别在4℃和25℃条件下，将雄蜂滞育蛹与F2杂交体孵育100 d，比较测定其血淋巴中海藻糖含量和过冷点。在25℃和4℃孵育的稻瘟病菌蛹中，海藻糖含量分别为15mg/ml和34mg/ml。在25℃和4℃孵育的F2代蛹中，平均含量分别为14mg/ml和18mg/ml。在4℃孵育的F2蛹中，海藻糖含量在5 ~ 37 mg/ml之间变化。25℃孵育蛹的过冷点平均为-27℃，4℃孵育蛹的过冷点平均为-21℃。F2个体的过冷点与糖含量无相关性。根据我对回交后代的观察，明确了玉米的绿色皮肤颜色是由常染色体上的显性基因控制的。黑斑螟皮肤黑斑也由另一个常染色体上的显性基因控制。S. c . pryeri的茧是棕色的。在B1代进行茧色分离，观察到各种颜色。一些基因分析让我用一个模型来解释茧的颜色；一个基因和另一个基因分别控制一种红色色素和一种灰色色素。

The F1 hybrids were obtained from a cross between Samia cynthia pryeri ♀× Samia cynthia ricini ♂. The F1 female pupae did not enter diapause, whereas most of the F1 male pupae enter diapause, suggesting that the pupal diapause nature is inherited in a sex linked manner. In the backcross progeny, the pupal diapause seemed to be sex linked. In F1 and B1 generations, the pupae did not show diapause under a long day photoperiod. The diapausing pupae of S. c. pryeri and the F2 hybrids were incubated at 4℃ vs. 25℃ for 100 days, and their trahalose content and supercooling point in the hemolymph were measured comparatively. In 25℃- and 4℃-incubated pupae of S. c. pryeri, the trehalose content was 15mg/ml and 34mg/ml, respectively. In 25℃- and 4℃-incubated pupae in the F2 generation, it was 14mg/ml and 18mg/ml (average), respectively. In 4℃-incubated pupae in the F2, trehalose content varied from 5 to 37 mg/ml among individuals. The supercooling point was -27℃ in the 25℃-incubated pupae and -21℃ at 4℃-incubated ones, in average. No correlation was observed between the supercooling point and trahalose content in F2 individuals.Based on my observation of the backcross progenies, it was clarified that the green skin color in S. c. pryeri was controlled by a dominant gene on an autosome. The black spots on the skin of S. c. pryeri was also controlled by a dominant gene on another autosome. The cocoon of S. c, pryeri was brown. The cocoon color was segregated in the B1 generation, and various kind of colors were observed. Some genetical analyses led me to explain the cocoon color by a model ; A gene(s) and another gene(s) control a red pigment and a gray pigment, respectively.