Studies on Germination Stimulants for Root Parasitic Weeds Produced by Root Cultures of Non-host Plants

Tissue culture techniques employed to explore capabilities of several plants to produce Strigagermination stimulants. The techniques used for The first time and offered several advantagesover the commonly used conventional techniques of growing plants hydroponically.他们并不多laborious, required less spaceallowed control of the environment and eliminated problems caused by microorganisms. Furthermore，as in the case of several other secondary metabolites from tissue cultures by manipulation of mediumcomposition.Menispermum dauricum root cultures were the most efficient producer of Strigagermination stimulants compared across all plants tested. The cultures produced at least 3 activecompounds. High-stimulant-producing tissue cultures were established by manipulation of cultureconditions. A modified B5 medium containing 35.7mM nitrogen at ANO - D23 - D2 - D1/NH - D24 - D2 - D1+ D1 ratio of 1：42,0.1 mM Fe y D12+ y D1, 1.0 mM Ca y D12+ y D1, 0.55 mM inorganic phosphorus, 0.28 mM inositol，μM nicotinic acid, 3.7 μM pyridoxine hydrochloride, 14.8 μM thiamine hydrochloride, 1 μM NAA，and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% andstimulant production by 5-fold，in comparison to the standard B5 medium supplemented with 3% sucrose and 1 μM NAA.The major Strigagermination stimulant produced by M. dauricum根cultures displayed similar chromatographicbehavior,similar UV和mass spectra to authentic strigol. Accordingly the major Striga germination stimulantproduced by M. dauricum root cultures was most likely strigol or a strigol-like molecule。

Tissue culture techniques were employed to explore capabilities of several plants to produce Striga germination stimulants. The techniques were used for the first time and offered several advantages over the commonly used conventional techniques of growing plants hydroponically. They were less laborious, required less space, allowed control of the environment and eliminated problems caused by microorganisms. Furthermore, as in the case of several other secondary metabolites from tissue cultures by manipulation of medium composition.Menispermum dauricum root cultures were the most efficient producer of Striga germination stimulants compared across all plants tested. The cultures produced at least 3 active compounds. High-stimulant-producing tissue cultures were established by manipulation of culture conditions. A modified B5 medium containing 35.7mM nitrogen at a NOィイD23ィエD2ィイD1-ィエD1/NHィイD24ィエD2ィイD1+ィエD1 ratio of 1 : 42, 0.1mM FeィイD12+ィエD1, 1.0 mM CaィイD12+ィエD1, 0.55 mM inorganic phosphorus, 0.28 mM inositol, 4.1 μM nicotinic acid, 3.7 μM pyridoxine hydrochloride, 14.8 μM thiamine hydrochloride, 1 μM NAA, and 4% sucrose sustained root growth for a longer period and increased root biomass by >30% and stimulant production by 5-fold, in comparison to the standard B5 medium supplemented with 3% sucrose and 1 μM NAA.The major Striga germination stimulant produced by M. dauricum root cultures displayed similar chromatographic behavior, similar UV and mass spectra to authentic strigol. Accordingly the major Striga germination stimulant produced by M. dauricum root cultures was most likely strigol or a strigol-like molecule.