Study on the flower color change of morning glory by change of vacuolar pH

花色素花青素在体外会随着pH值的变化而不断变色，其中一个原因是花色素存在于细胞内的液泡中，其颜色的变化是不同的。为定量阐明花瓣液泡pH值与花色的关系，发展和建立了细胞内微电极法直接测定花瓣液泡pH值的方法。构建了微电极法测定花瓣液泡内离子浓度的电生理实验台。除pH电极外，还进行了填充钾离子载体的细胞内微量钾响应电极的制备。测定了蓝色牵牛花(Ipomoea tricolor，cv.天蓝)、红色牵牛花(Pharbitis nil cv.Scarlet O‘Hara)花瓣的液泡pH值。红蕾花瓣液泡的pH值约为6.6，开放花的pH值为7.6，而红色牵牛花的液泡pH值约为6.1，远低于天蓝色牵牛花的7.6。牵牛花紫色花瓣细胞液泡的钾离子浓度为70 mM，蓝色开放花的液泡钾离子浓度为38 mM。花色与液泡离子浓度的关系尚不清楚，研究正在进行中。

The flower color pigment anthocyanin changes its color continuously in vitro by the change of pH.One of the the causes of this the color change of the flower variously will be a difference of vacuole pH in which the pigment exists intracellular. This study was done for the purpose of quantitatively clarifying the correlation of vacuole pH and flower color by developing and establishing direct measurement method in this study of petal vacuole pH by the intracellular micro-electrode method.Electrophysiology loboratory-table for the ion concentration measurement in the petal vacuole by the micro-electrode method was constructed. It also carried out besides besides pH electrode preparation of intracellular minute potassium responsibility electrode which filled the potassium ionophore.The vacuolar pH of Blue morning glory (Ipomoea tricolor, cv.Heavenly blue), red Japan morning glory (Pharbitis nil cv.Scarlet O'hara) petals were measured. Though in the red buds petals vacuole pH was about 6.6, the pH value of open flower was 7.6 The vacuolar pH of the red morning glory was about 6.1, and it was proven to be considerably lower than 7.6 of the sky-blue morning glory.Potassium ion concentration of the purple petal cell vacuole of the morning glory was 70 mM.The ivacuolar potassium ion concentration of blue opened flower was 38 mM.The relationship between flower color and ion concentration in the vacuole has much ineterst and the study are under progress.