Studies on the production of prostaglandin E2(PGE2) and its related substances in red algae of the genus Gracilaria

在日本有三起官方中毒事件，包括三起死亡，因此，对日本的格蕾西利亚原始红藻的意义。1993年10月，食品中毒事件发生到“ogonori”（Gracilaria Verrucosa）的意义，在横滨和Kanagawa Prefecture的一个明显的红色藻类。日本，两个受害者的结果，包括一个死亡（女性）。在蘑菇中没有因果性的试剂是从海洋毒素的例行生物测定中发现的。Responsibility of prostaglandins（PGs）、particularly mainly PGE2、for the“ogonori”poisoning has been suggested。这种类型的中毒非常不寻常，而且在海洋组织的种植后，出现了更多的常见形式的中毒差异。从一个食品健康的角度来看，PGE 2的生产及其相关物质对五种类型的红藻进行了审查，如G. verucosa、G. Bursa-pastoris、G. Gracilariopsis chorda、G.G.。日本Seto内陆海的textorii和Gracilaria sp.在这个研究项目中。在G. verucosa的一种特殊物质中，PGE 2在新鲜海藻中发现了0.1-57.9微克/克，而且增加了氢化物，其数量从1.8 - 90微克/克不等。从该特定物中处理的液体硝酸盐的增加PGE 2数量的产量在与未暴露的Speoimen的比较中有几个更大的时间。然而，PGE 2的生产被极大程度地降解为藻类的酸治疗。在红藻类中，除了G. verucosa，PGE 2没有被检测到。However，未识别的PGE 2相关物质被检测到，任何紫外线吸收最大值接近240纳米。其中之一将上升到一个积极的ESI质量光谱中，展示了m/z372上的（M+H）y D1+ y D1和其他分子的保护分子。（M+H-H型D22型D2 O）Y型D1+ Y型D1 at m/z355，（M+ H-2 H-D22 D）D-1 + D-1 at m/z337， （M+ H-3 H-2 D-2 O）D-1 + D-1 at m/z319 and （M+ H-4 H-2 D-2 O）D-1 + D-1 at m/z301。

There have been three official poisoning incidents in Japan, including three deaths, due to the ingestion of raw red algae of the genus Gracilaria have occurred in Japan. In October 1993, a food poisoning incident due to the ingestion of 'ogonori'(Gracilaria verrucosa), an edible red alga occurred at Yokohama, Kanagawa Prefecture. Japan, resulting in two victims, including one death (female). No causative agent present in the ogonori was found from a routine bioassay for marine toxins. Responsibility of prostaglandins(PGs), particularly mainly PGE2, for the "ogonori" poisoning has been suggested. This type of poisoning is very unusual, and differs from the more familiar forms of poisoning occurring after ingestion of marine organisms.From a food hygienic point of view, the production of PGE2 and its related substances was examined in five species of red algae such as G.verrucosa, G.bursa-pastoris, Gracilariopsis chorda, G. textorii and Gracilaria sp. in Seto Inland Sea, Japan in this research project. In the specimens of G.verrucosa, 0.1-57.9μg/g of PGE2 was found in fresh alga and, on addition of araohidonate, its amount increased from 1.8 to 90μg/g. The production of increased amount of PGE2 from the specimens treated with liquid nitrogen was several times larger in comparison with that of untreated speoimens. But the production of PGE2 was extremely decreased by acid treatment of the algae. In the red algae except for G.verrucosa, PGE2 was not detected. However, unidentified PGE2 related substances were detected, whose UV absorption maxima was near 240nm. One of these gave rise to a positive ESI mass spectrum, showing a protonated molecule ion of (M+H)ィイD1+ィエD1 at m/z 372 and other ions of (M+H-HィイD22ィエD2O)ィイD1+ィエD1 at m/z 355, (M+H-2HィイD22ィエD2O)ィイD1+ィエD1 at m/z 337, (M+H-3HィイD22ィエD2O)ィイD1+ィエD1 at m/z 319 and (M+H-4HィイD22ィエD2O)ィイD1+ィエD1 at m/z 301.