Follicular dendritie cells and RAG-1&RAG-2 expression on germinal center B cells.

生发中心B细胞(GCB)是滤泡树突状细胞(FDC)的一种新的功能标志物，FDC可通过这种反应调节GCB的凋亡易感性。在本研究中，我们旨在利用RT-PCR方法来明确FDC是否也调节GCB上RAG-1和RAG-2的重新表达。我们的研究仍在继续，直到最后的报告，因为在活体系统中，RAG-1和RAG-2在GCB上的重新表达的假设似乎是被接受的。我们的初步结果是：(1)扁桃体GCB上未检测到RAG-1和RAG-2的明确表达。(2)滤泡B细胞上经常可以诱导RAG-1和RAG-2的重新表达。(3)FDC不能调节这种再表达。(4)病理细胞系、滤泡性淋巴瘤(3/3)和部分弥漫性大B细胞淋巴瘤(中心母细胞1/9，富含T细胞/组织细胞的5/7)的RAG-1和RAG-2表达呈阳性(Nakamura等人)。总之，FDC可能不参与GCB中RAG-1和RAG-2的再表达。因此，应继续研究RAG-1和RAG-2在B细胞恶性过程中的作用。

In our previous project, we have reported that emperipolesis of germinal center B cells (GCB) is a novel functional marker of follicular dendritic cells (FDC) and FDC can modulate the susceptibility of apoptosis of GCB through this reaction. In this study, we aimed to clear using RT-PCR method whether FDC also regulate the re-expression of RAG-1 ＆ RAG-2 on GCB.We continue our research still for the final report, since the hypothesis seems to be accepted that the re-expression of RAG-1 ＆ RAG-2 on GCB is doubtful in vivo system. Our preliminary results are ; (1) The clear expression of RAG-1&RAG-2 could not be detected on tonsillar GCB.(2) The re-expression of RAG-1&RAG-2 could be often induced on follicular B cells. (3) FDC could not modulate this re-expression. (4) The survey of RAG-1&RAG-2 expression on the pathological cell lines, all follicular lymphoma (3/3) and some diffuse large B cell lymphoma (centroblastic 1/9, T cell/histiocyte-rich 5/7) showed positivity (Nakamura et al.)In summary, FDC might not be involved in the re-expression of RAG-1＆ RAG-2 on GCB.The examination of B-cell lines, however, suggests that RAG-1&RAG-2 expression have some relationship of malignant process within germinal centers. Therefor this research should be continued for clarify the role of RAG-1&RAG-2 on B cell malignant process.