6 Development and function of γδT cells

T细胞的两个类别，命名为T细胞表达T细胞受体-αβ（TCR-αβ）和T细胞表达TCR-γδ，已在所有vertebrates测试中识别。现在已经很清楚了，大多数αβ T细胞识别出的抗原片段的肽片段是由主要的历史相容性复合体分子所承担的，并且在最具特征的细胞介导的抗原特异性免疫反应中承担的。与此相反，γδ T细胞识别仍然未定义和免疫反应中γδT细胞的生物学roe不是很好地理解的。我们分析了体外T细胞的细胞活动（IEL）隔离的2-to 3-old mutant mice rendered in different gene（s）中不同基因（s）中的数目的IEL表示像TCR-αβ（αβ-IEL）或TCR-γδ（γδ-IEL）要么是空白的，要么是被标记的。当与野生类型（WT）小元素比较时，γδ-IEL在TCR-β突变体（β i-D1-/-i-D1）中共享受干扰但在TCR-α突变体（α）中保留的细胞活性 ... More イイD1-/-イエD1） mice.反CD 3和反TCR-γδ mAb-诱导IFN-γ生产的γδ-IEL显示了相同的TCR-α和TCR-β突变-依赖变异性。与β i D1-/-ye D1老鼠，α i D1-/-ye D1老鼠预先放置在抗体中产生B细胞保密的自动抗体和形成细胞中心。在事实上，这些特征是可以比较或甚至超过那些年龄匹配的wt老鼠和淋巴细胞质量在α-D1-/--D1老鼠变得大于那些在控制wt老鼠之后暴露在环境抗原或年龄。Our present study revealed that， even during 2-3 months of young adult age， cytolytic and IFN-γ-producing activities of γδ-IEL were uniformly high in α伊D1-/-D1 mice but very low in β伊D1 mice.从2到3个月的旧α D1-/-β D1和β D1-/-β D1老鼠被标记为它们也是以前的细胞质，但不在最近的突变老鼠中，在此上下文中，持久的colonization of constitutively activated Thy-1-D1+-IEL subset in the intestinal epithelia of young--D1-/-您的位置：知道173>> D1 mice for the subsequent Management of inflammatory bowel disease （IBD） in older D1-/-D1 mice。因此，不仅是β-D1 dim-D1 T cells，但也可能是激活γδ T cells in the intestinal mucosa，可能扮演一个重要的角色在早期阶段的慢性IBD的发展。Less（低）

Two classes of T cells, namely, T cells expressing T-cell receptor-αβ(TCR-αβ) and T cells expressing TCR-γδ, have been identified in all vertebrates examined to date. It is now clear that most αβ T cells recognize peptide fragments of antigens presented by major histocompatibility complex molecules and are engaged in most characterized cell-mediated antigen-specific immune responses. In contrast, the general rules forγδT-cell recognition remain undefined and the biologic roe ofγδT cells in immune responses is not well understood.We analyzed the cytolytic activity of intraepithelial T cells (IEL) isolated from the small intestines of 2- to 3-month-old mutant mice rendered deficient in different gene(s) in which the number of IEL expressing either TCR-αβ(αβ-IEL) or TCR-γδ(γδ-IEL) were absent or markedly diminished. When compared with wild-type (WT) littermates, cytolytic activity ofγδ-IEL was sharply attenuated in TCR-β mutant (βィイD1-/-ィエD1) mice but remained unaltered in TCR-α mutant (α … More ィイD1-/-ィエD1) mice. The anti-CD3 and anti-TCR-γδ mAb-induced IFN-γ production of γδ-IEL showed the same TCR-α and TCR-β mutation-dependent variability.In contrast to βィイD1-/-ィエD1 mice, αィイD1-/-ィエD1 mice are predisposed to a marked increase in antibody producing B cells secreting autoantibodies and to germinal center formation. In fact, the traits are comparable to or even exceed those of age-matched wt mice and the cellular mass of lymphoid tissues in αィイD1-/-ィエD1 mice becomes greater than that in control wt mice, either after exposure to environmental antigens or with age. Our present study revealed that, even during 2-3 months of young adult age, cytolytic and IFN-γ-producing activities of γδ-IEL were uniformly high in αィイD1-/-ィエD1 mice but very low in βィイD1-/-ィエD1 mice. Analysis of large intestinal γδ-IEL from 2- to 3-month-old αィイD1-/-ィエD1 and βィイD1-/-ィエD1 mice indicated that they were also cytolytic in the former but not in the latter mutant mice, In this context, the persistent colonization of constitutively activated Thy-1ィイD1+ィエD1 γδ-IEL subset in the intestinal epithelia of young αィイD1-/-ィエD1 mice probably has important implications for the subsequent development of inflammatory bowel disease (IBD) in older αィイD1-/-ィエD1 mice. Thus, not only the βィイD1dimィエD1 T cells, but also possibly the activated γδ T cells in the intestinal mucosa, may play an important role in the early stages of development of chronic IBD in αィイD1-/-ィエD1 mice. Less