Genetic alterations in cancers of digestive tract as analyzed by CGH, in relation to genesis of cancer and the metastasis.

从1994年到1997年，我们用比较基因组杂交(CGH)方法分析了胃癌切除标本的DNA拷贝数变化，发现不同分化程度和肿瘤分期的DNA拷贝数变化模式不同(临床癌症研究3：1067-1076,1997)。在这项研究中，我们通过检测根除幽门螺杆菌前后的低度恶性淋巴瘤组织，进一步扩展了这些发现。根据内窥镜和组织学观察，根除导致恶性淋巴瘤完全或部分消退。为了了解这种“回归”是否也发生在DNA水平上，对样本进行了CGH分析。我们发现了DNA异常仍然保留的病例，尽管在根除后判断为正常。我们也在分析结肠癌和胃癌的癌前病变，以及肝细胞癌，以寻找肿瘤形成初期的基因变化。为了评估在胃癌中获得的结果，我们还分析了肾上腺皮质肿瘤的基因变化与恶性程度和激素产生的关系，这取决于我们通过CGH发现变化模式的不同。最后，我们检测了口腔鳞状细胞癌的遗传改变，发现在1q、3q、5q、8q、11p、11q、13q和20p处扩增，而在18q处DNA拷贝数减少。

From 1994 to 1997, we analyzed resected gastric cancer samples for the DNA copy number alterations by CGH (Comparative Genomic Hybridization) and found differences in pattern of alterations depending on the extent of differentiation and tumor stage (Clinical Cancer Research 3 : 1067-1076, 1997). In this research, we have further extended these findings by examining low grade MALToma tissues before and after eradication of H. pylori. The eradication resulted in complete or partial regression of MALToma judging from endoscopic and histological observations. To see whether this "regression " also occurred at DNA level, the samples were subjected to CGH analysis. We found cases where DNA abnormality was still retained, though judged to be normal after the eradication. We are also analyzing precancerous lesions of colon and gastric, and hepatocellular carcinomas to find genetic alterations at initial stages of tumorigenesis.To evaluate the findings obtained in gastric cancers we also analyzed adrenocortical tumors for genetic alterations in relation to extent of malignancy and homone production, depending on which we found differences in alteration patterns by CGH. Finally, we have examined genetic alsterations in oral squamous cell carcinomas and found amplifications in 1q, 3q, 5q, 8q, 11p, 11q, 13q, and 20p while decrease of DNA copy number in 18q.