Induced HSP 70 by pre-heating protect the living body

高热的目的是治疗癌症。由于热休克蛋白70是高热耐热的原因之一，因此热休克蛋白70是癌症治疗的负担。本研究的目的是建立一种诱导大量热休克蛋白70而不产生热损伤的预热方法，并确定预热诱导的热休克蛋白70的细胞保护作用，以保护活体免受手术、休克、缺血、感染等的伤害。第一年考察预热的方法，第二年考察预热诱导的HSP70的活体防御效果。预热试验对无活体无序、产生大量热休克蛋白的预热方法进行了试验。小鼠以40℃恒温30min，重复1、2、3、4次，用ELISA法测定部分脏器和淋巴细胞中HSP70的浓度。加热诱导了大量的HSDP70，每个…最多一个更多的器官和淋巴细胞。2.热休克蛋白70诱导的GY预热对内毒素休克小鼠的保护作用：预热后1d组、2d组、3d组、7d组分别注射℃，检测小鼠的存活率。观察未预热对照组小鼠的存活率及小鼠存活率。未预热对照组的存活率为23%，1d组为30%，2d组为45%，7d组为20%。预热后2天的存活率是对照组的2倍，说明预热诱导的HSP70具有保护作用。预热诱导的热休克蛋白70对热致小鼠下肢肌肉疲劳的保护作用小鼠肢体预热温度为41℃，预热时间为30min。小鼠肢体在48℃的温度下加热30min。在预热后1、2或7天。用31P-MRS测定肢体肌肉的能量代谢。未预热的对照组小鼠能量耗竭时间为40min，1d组为60min。2天组超过60min。7d组能量耗竭时间为40min，与对照组相同。结果表明，预热诱导的HSP70对机体的内毒素休克、DIC衰竭、缺血、手术、感染等有一定的保护作用。较少

The purpose of the hyperthemia is cancer treatment. Because HSP 70 is one of the causes of thermotolerance with hyperthemia,HSP 70 is a burden for the cancer therapy. The purpose of this research is to establish a method of pre-heating which induces a large amount of HSP 70 and don't give a heat injury, and to confirm the cytoprotective action of HSP 70 induced by pre-heating defend the living body from the operation, shock, ischemia, infection and so on. The method of the pre-heating was examined in the first year, and the living body defence effect of HSP 70 induced by pre-heating was examined in the next year.1. The examination of pre-heatingThe method of pre-heating which gave no disorder in living body and induced a large quantity of HSP was examined. Mice were heated 1,2,3 and 4times of the repetition with whole-body at 40℃for 30min and concentration of HSP 70 in some organs and lymphocytes was measure by ELISA method. Heating induced a large quantity of HSDP 70 most one in each … More organs and lymphocytes. It was decided that the pre-heating method, rectal temperature 40℃ for 30 min with whole-body.2.The protective effect of HSP 70 induced gy pre-heating for the endotoxin shockMice were injected LPS on (1day group), 2(2days group), 3(3days group) or 7 days group) after pre-heating and the survival rate of the mice was examined. The survival rate of the control group which didn't pre-heating was and the survival rate of the mice was examined. The survival rate of the control group which didn't pre-heating was 23%, 1day group was 30%, 2 days group was 45% and 7 days group was 20%. The survival rate on 2 days after preheating was two times of the control group, and the protective effect of HSP 70 induced by pre-heating was shown.3. The protective effect of HSP 70 induced by pre-heating for the muscular exhaustion of the murine inferior limb with heatingThe mice limb was pre-heated at 41℃for 30 min. The mice limb was heated at 48℃for 30min. on 1,2 or 7 days after pre-heating. The energy metabolism of the limb muscle was measured with 31P-MRS. Energy exhaustion time of the control mice which didn't pre-heating was 40min, that of 1day group was 60min. And 2days group was more than 60min. The energy exhaustion time of 7 days group was 40min, in the same way as the control group. The exhaustion of the inferior limb muscle of mice was retarded by the pre-heating.From these results, it was proved that HSP 70 induced by pre-heating prevents the living body from such as endotoxin shock, DIC exhaustion, ischemia, operation, infection and so on. Less