C-11 acetate PET measurement of hypoxia : Correlations with radio sensitivity and multidrug resistance

癌细胞C-11乙酸清除率可能反映了癌细胞的氧化代谢。本研究的目的是评估C-11醋酸盐清除率与肺癌放射敏感性或多药耐药之间的相关性。FDG摄取，Tc-99 mMIBI清除率和多药耐药之间的相关性也进行了评估。进行动态C-11乙酸盐PET研究以估计C-11乙酸盐从肺癌的清除。注射后40 min进行FDG PET。注射后15 min和3 h分别行~（99）Tc-MIBI SPECT显像。全量放疗后4周行CT检查评价放射疗效。多药耐药通过免疫组织化学染色使用抗P-糖蛋白抗体（JSB-1）进行评估。C-11醋酸盐清除率与肺癌放射敏感性及P-糖蛋白表达无明显相关性。然而，FDG摄取与P-糖蛋白表达之间存在显着相关性。P-糖蛋白强表达的肺癌表现为低FDG摄取。Tc-99 mMIBI清除率与P-糖蛋白表达相关。强P-糖蛋白表达的肺癌表现出较高的Tc-99 mMIBI清除率。这些结果表明，FDG摄取和Tc-99 mMIBI清除可能反映了肺癌P-糖蛋白的表达。

C-11 acetate clearance from cancer cell may reflect oxidative metabolism of cancer cell. The purpose of this study was to assess correlations between C-11 acetate clearance and radiosensitivity or multidrug resistance in lung cancer. Correlations between FDG uptake, Tc-99m MIBI clearance and multidrug resistance were also assessed. Dynamic C-11 acetate PET study was performed to estimate C-11 acetate clearance from lung cancer. FDG PET was performed 40 min after injection. Tc-99m MIBI SPECT was performed 15 min and 3 hrs after injection. Radiation effect was estimated by CT study 4 weeks after full dose radiotherapy. Multidrug resistance was estimated by immunohistochemical staining using anti-P-glycoprotein in antibody (JSB-1). There were no significant correlations between C-11 acetate clearance and radiosensitivity or P-glycoprotein expression in lung cancer. However, there was a significant correlatitween FDG uptake and P-glycoprotein expression. Lung cancer with strong P-glycoprotein expression showed low FDG uptake. The Tc-99m MIBI clearance correlated with P-glycoprotein expression. Lung cancer with strong P-glycoprotein expression showed high Tc-99m MIBI clearance. These results indicate that both FDG uptake and Tc-99m MIBI clearance may reflect P-glycoprotein expression in lung cancer.