A Nobel Vascular Prosthesis Graft Using Acellular Xeno- Graft

目前还没有报道的血管假体具有生物学上适合宿主、生长和重塑的能力。缺乏这样的移植物限制了患者，特别是儿科患者的长期利益。作为迈向理想移植物的第一步，我们研究了一种由无细胞基质组成的新型血管假体，以评估假体上宿主组织再生的程度。将直径为6-8 mm的猪颈动脉用1%Triton X-100洗涤剂处理48小时。移植物表面肝素化。这种去细胞血管假体植入4只成年比格犬的腹主动脉。分别于术后20天和3~51周处死动物，行血管造影术，光镜和免疫组织化学检查显示，植入前动脉核已从假体中完全摘除。所有植入的移植物均通畅，无管壁不规则。术后3周，除中段外，移植物内腔均被内皮细胞覆盖。在18周时，所有移植物的整个表面都被内皮细胞覆盖。扫描电子显微镜检查显示，内皮细胞沿移植物的纵向排列在管腔表面，利用新型无细胞异种血管假体可实现宿主组织的再生。

There is no reported vascular prosthesis that has the ability to biologically fit the host, grow and remodel. Lack of such grafts limits the long-term benefits for patients especially pediatric ones.As a first step toward the ideal graft, we examined a novel vascular prosthesis that consists of acellular matrix to evaluate the degree of the host tissue regeneration on our prosthesis.A porcine carotid artery with a diameter of 6-8mm was treated with 1 % detergent Triton X-100 for 48hours. The graft surface was heparinized. This acellular vascular prosthesis interposed the abdominal aorta in 4 adult beagle dogs. Animals underwent angiography and were sacrificed 20 days and 3-51 weeks after the surgery, respectively.Microscopic and immunohistochemical examination revealed that the nuclei of the artery were completely removed from the prosthesis before implantation. All the implanted grafts were patent without the irregularity of its wall. At 3 weeks postoperatively, the internal lumen of the graft was covered with the endothelial cells except the mid portion. At 18 weeks, the entire surface of all the grafts was covered with the endothelial cells. The SEM examination revealed that the endothelial cells were lined on the luminal surface in the longitudinal direction of the graft.Regeneration of the host tissue may be accomplished using the novel acellular xeno-vascular prosthesis.