Then analysis of lung cancer protein using two-dimensional polyacrylamide gel electrophoresis Application for adjuvant chemotherapy

1. 用二维聚丙烯酰胺凝胶电泳法检测原发性肺癌组织病理分化相关的16个多肽。根据与组织病理分化或细胞增殖相关的多肽的表达，我们将原发性肺腺癌分为典型病例和非典型病例。65.4%的典型病例病理诊断为高分化腺癌。65.4%的典型病例淋巴结转移呈阴性。非典型病例中分化良好者占20.5%，无淋巴结转移者占34.9%。此外，我们发现3年无病生存率有统计学意义（典型病例62.9%，非典型病例27.1%,p=0.0011）。制备了TA02分子n端单克隆抗体。利用该抗体与人体组织进行免疫组化分析，明确了TA02的组织分布。此外，我们还获得了TA02分子与1998年底发表的一种新型天冬氨酸蛋白酶NapsinA同源的证据，因为高表达TA02的腺癌病例在RNA水平上表达napsin a。此外，Napsin A的GST融合蛋白与抗ta02抗体呈阳性反应。与神经内分泌细胞相关的TS02与蛋白基因产物9.5（PGP 9.5）同源。TS02阳性患者的2年预后较TS02阴性患者差。比较耐cddp细胞株与亲本细胞株之间的2DE图谱，确定了网绿卡宾和谷胱甘肽- s -转移酶π在2DE凝胶上的位置。内质网钙结合蛋白reticulocalbin在耐cddp细胞系中的表达水平降低。另一方面，GST-π的表达水平显著升高。基于肺癌蛋白的2DE分析对生物肿瘤恶性程度的评价具有重要价值。少

1. Sixteen polypeptides associated with histopathological differentiation were detected using two-dimensional polyacrylamide gel electrophoresis of primary lung carcinoma.2. Based on the expression of the polypeptides associated with either histopathological differentiation or cell proliferation, we classified primar lung adenocarcinoma into typical case and atypical case. 65.4% of the typical cases were diagnosed as well differentiated adenocarcinoma histopathologically. Also, 65.4% of the typical cases showed negative in lymph node metastasis. On the other hand, 20.5% and 34.9% of the atypical cases were judged as well differentiated case and cases without lymph node metastasis, respectively. Furthermore, we recognized the statistically significant difference in three year-disease free survival(62.9% in typical cases, 27.1% in atypical cases ; p=0.0011).3. We produced monoclonal antibodies against N-terminal of TA02 molecule. The histological distribution of TA02 was clarified immuno … More histochemically using this antibody and human tissues. Also, we obtained the evidence that TA02 molecule was homologos with NapsinA which was a novel aspartic proteinase published at the end of 1998, because the adenocarcinoma cases with high expression of TA02 showed the expression of napsin A in RNA level. Furthermore, GST fusion protein of Napsin A showed positive reaction with anti-TA02 antibody.4. TS02 associated with neuroendocrine cells was homologous with protein gene product 9.5(PGP 9.5). In the TS02 positive cases two-years prognosis become worse compared to TS02 negative cases.5. Compred to the 2DE patterns between CDDP-resistant cell line and the parents cell line, the positions of reticulocarbin and glutathione-S-transferase π were identified on 2DE gel. The expession level of reticulocalbin, which was Ca-binding protein at endoplasmic reticulum, became low in the CDDP-resistant cell line. On the other hand, the expression level of GST-πbecame high, remarkably.6. The analysis based on lung cancer protein using 2DE is valuable for the evaluation of biological tumor malignancy. Less